Development and Assessment of Carbon Nanotube-Curcumin Modified Nanoconjugate System for Breast Cancer Targeting

Abstract

The objective of the current research was to formulate SWCNTs anchored curcumin utilizing adipic acid dihydrazide (ADH) via carbodiimide conjugation for efficient targeting against human breast cancer celllines (MDA-MB-231). The CNT- C conjugate was formulated and evaluate the conjugation pattern by FTIR analysis. The system was also be characterized by various parameters such as zeta potential, particle size, transmission electron microscope (TEM), X-ray diffraction analysis, drug loading, drug release, in-vitro anticancer, apoptosis and haemolytic toxicity assay. The surface charge and particle size of the system was found to be -19.5 mV and 178.21 nm respectively. According to the result obtained from morphological assessment (TEM), the CNT was found in nanometric size range and remain in separate form, no adherence was also be obtained in analysis. The release of curcumin from CNT was found to be 58.36% after 120 h period of time at 7.4 pH. The release rate of curcumin from CNT was found 62.35% and 69.58% in case of 5.5 pH and 4.5 pH after 120 h. As per the result of in-vitro cytotoxicity assay, it was found that, with increasing the concentration of nanocomposites the cell growth was inhibited and the nacrotic cell population was increased from 3.25% to 18.98% obtained from apoptosis assay. The Overall finding obtained from the above analysis it was suggested that the CNT system was found in nanometric size range and having superior drug loading efficiency. The in-vitro anticancer assay suggested the anticancer potential of the CNT system and the data obtained from apoptosis assay, it was exposed that the system may successfully enhanced the population of nacrotic cells in comparison with control and should be a potential agent for cancer treatment.