Unveiling the Synoptic Research of in Vitro Pharmacological Activities in Methanolic Extracts of Both Fruits and Roots of Coccinia Grandis

Abstract

Ethnopharmacological relevance: Coccinia grandis is well known for its curative properties and used as folk medicine in India and other countries due to its various pharmacological characteristics due to presence of number of phytochemicals.

Aim: The aim of the present study was to evaluate pharmacological properties of the fruits and roots of Coccinia grandis.

Materials and methods: The methanolic extracts of these parts were tested and analyzed for antibacterial, anti-inflammatory, anti-oxidant, cytoprotective and antidiabetic properties. The antibacterial activity of both extracts was tested against two strains of E.coli and Staphylococcus aureus using agar diffusion method. The in vitro anti-inflammatory activity was done using RBC membrane stabilization method and inhibition of protein denaturation method. The anti-oxidant analysis was done using DPPH, FRAP, SOD, nitric oxide radical scavenging assay. Cytoprotective activities were carried out by MTT assay in 24 hr – HeLA cells. The anti-diabetic activity was done by inhibition of alpha amylase method and non enzymatic glycosylation of hemoglobin method. Results: The methanolic extracts of both roots and fruits had showed variety of phytochemicals alongwith antioxidant, antibacterial, anti-inflammatory, anti-diabetic and cytoprotective activities. However, fruit extract showed greater activities as compared to root extract.

Conclusion: Methanolic fruit extract can serve as promising source for various pharmacological activities.