Development and Characterization of Luliconazole and Chamomile Oil Loaded Nanoemulsion based Gel for Effective Treatment of Fungal Disease

Abstract

Luliconazole (LNZ) is a selective, poorly water-soluble (0.000304mg/ml) azole effective against treatment of fungal infections when administered in doses. In the present Research work, to formulate nanoemulsion of luliconazole using chamomile oil for the treatment of fungal disease. Chamomile oil chosen as oil as it is known for its antifungal activity. The nanoemulsion system was optimized through D-optimal design and an equivalent amount of nanoemulsion is incorporated in Carbopol 934p based gel. Optimized nanoemulsion contained 33.13% Smix, 9.05% oil, 57.80% water which has smallest globule size of 140 nm and PDI was 0.367. Zeta potential was obtained as -14.6 mV. Viscosity of nanoemulsion and nanoemulsion based gel, 128 cP and 1380 cP respectively and pH of nanoemulsion was 7.25. All nanoemulsion were O/W type as per results of conductivity which is 84.2 µs/cm. For nanoemulsion based gel, ex vivo study revealed that drug release at 5 hours was 69.48%. and an in vitro study release 89.21 % drug release. The range of antifungal action was increased by combining chamomile oil with Carbopol 934p based gel of luliconazole Via its zone of inhibition. The antifungal activity of a gel based on nanoemulsion was compared to a marketed formulation. Observations led to conclude that the 0.4% concentration of NEBG had a greater zone of inhibition than other commercially available formulations. zone. Thus, 0.4% of NEBG was shown to be more efficacious than other commercially available formulations, and that too with a relatively modest dosage of luliconazole.