Investigation of Phytochemical Potential of Various Extracts of Ipomoea Reniformis Chois

Abstract

This study aimed to provide pharmacological evidence of chloroform (CE), Ethyl Acetate (EA), Ethanol (EE) and Hydroalcoholic (HE) extract of Ipomoea Reniformis Chois (Convolvulaceae) preventing or healing peptic ulcers. The shade dried leaves of Ipomoea reniformis was extracted using Soxhlet apparatus. The extracts were subjected to determination of physical constants and phytochemical tests, Acid neutralizing capacity and Antioxidant capacity by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and Superoxide Radical Scavenging Activity. The extracts were characterization by thin layer chromatograms. Isolated compound were analyzed by UV, IR, NMR and Mass spectroscopy. The results showed total solid content 98.85 ±0.04 which is highest in physical constant, the percent yield was found  11.06 % highest for ethanol extract. All four extracts showed the presence of Glycosides, Flavonoids and Carbohydrates in abundance. Total phenolic content was highest 28.12 ±1.08 for EE extract while 6.21 ±0.13, 21.40 ±0.32, 16.84 ±0.19 CE, EA and HE presented unit milligrams of Gallic acid equivalent respectively. Total Flavonoids content was highest in EA 59.29 ±1.87 and EE 59.09 ±1.87 while CE has 52.32 ±0.69 and HE 24.54 ±1.87 in mg of RU/g of extract equivalent. The bioactive compounds identified in Ipomoea reniformis were correlated with their potential role in antiulcer activity based on the in vitro studies. To further confirm the identity of the active compounds, advanced techniques such as Nuclear Magnetic Resonance (NMR) spectroscopy, Mass Spectrometry (MS), and Fourier Transform Infrared (FTIR) spectroscopy were employed. These techniques allow for the structural elucidation of isolated bioactive compounds.