Extraction and Characterization of Glycerides from Oil Seed of Citrus maxima Plant found in Manipur

Abstract

Extraction of seed oil (Citrus maxima) was done by Solvent extraction technique on the crushed kernel using petroleum ether as the solvent. The oil was purified prior to further analysis by column chromatography over silica gel (60-120 mesh) using a mixture of petroleum ether and ethyl acetate (20:1) as the eluent. The purified oil was further trans esterified giving the final product known as biodiesel. The biodiesel is also known as the fatty acid methyl ester (FAME). Fatty acid methyl ester (FAME) composition of Citrus maxima seed oil was determined by NMR, IR and GC-MS analysis. The FAME of the oil of Citrus maxima was found to be 32.90 wt.% of methyl palmitate [C16:0], 29.70 wt.% of methyl linoleate [C18:1], 30.40 wt.% of methyl oleate [C18:1] and 7.00 wt.% of methyl stearate [C18:0]