Bio Changes Related with Cutaneous Leishmaniasis Patients in Babil Governorate.

Abstract

The study was performed to evaluate the histological changes in ulcers of infected patients with cutaneous leishmaniasis in Babil governorate/ Iraq. The study was performed in El-Sadeq hospital, Al-kifel hospital, Musaib hospital and Alexandria hospital within Governorate of Babil from 1/9/2021 to 1/4/2022. In our study we used full thickness biopsy stained with (H&E) and direct smear stained with Geimsa stain technique taken from each ulcer of 22 patients infected with CL. We could identify amastigote stage of the parasite in 8 of 22 patient (36%) by full thickness biopsy and (H&E) stain and in 10 of the 22 (45%) infected patients using direct smear stained with Geimsa stain. We could identify amastigote in one slide in the epidermis which is considered a rare finding. We could identify a heavy inflammatory cell in filteration in the superficial dermis which is considered a diagnostic feature by some authors in the absence of amastigotes in the lesion. Various studies were done to define the best technique to obtain the outmost results to identify the parasite and characteristic features in a smear or biopsy of the lesion.