Chemical Characterization of Siddha Poly Herbal Formulation Amurthathi Chooranam by Using Modern Analytical Techniques

Abstract

Background: Amurthathi Chooranam(AMC) is a Siddha poly herbal formulation mentioned in the Siddha text “AnubavaVaithiya Deva Ragasiyam” which is indicated for Urolithiasis. The drug Amurthathi Chooranam was prepared as per the standard operative procedure and it exposed to several studies to reveal for its effectiveness. Standardization of Siddha poly herbal formulations is mandatory to assess the quality of the drugs for treatment procedures.

Aim: The aim of the study was to standardize the AMC by modern instrumental analytical techniques such as Inductively Coupled Plasma Optical Emission Spectroscopy (ICPOES), Scanning Electron Microscope (SEM) and Fourier Transform Infra-Red Spectroscopy (FTIR).

Materials and methods: FTIR and ICP-OES analysis was performed at IIT, Madras as per the standard procedure. SEM analysis was carried out as per the standard procedure at Anna University, Crystal Growth Centre, Chennai.

Results and Discussion: ICP-OES reveals high concentration of P in AMC (146.341 mg/L). It also has physiologically important minerals like Ca, Fe, K, Mg, Na, S and Zn. The heavy metals such as Mercury, Arsenic, Lead, Cadmium,and Aluminium were observed as Below Detectable Limit.The chemical finger print was exhibited by using modern analytical techniques like Fourier Transform Infra-Red Spectroscopy (FTIR).The stretches and bonds present in the FTIR analysis indicated the presence of functional groups Amide, Phenols, Alcohols, Alkanes, Aldehyde, Amine, Alkenes, Alkanes, Ester, Ether, Alkyne and Halo compounds which may be the reason for the therapeutic potency of the trial drugAMCand the SEM picture indicated the existence of microparticles and its bio availability. Based on the results, Amurthathi Chooranam is safe, preferably non-toxic to human for its therapeutic dose.