Estimation of Lercanidipine HCl By AUC of UV-VIS Spectrophotometry Technique & RP-HPLC Method

Abstract

An attempt was made to develop a validated method for the estimation of Lercanidipine HCl in bulk and its dosage form by using the area under curve and the RP-HPLC method. In Method 1, the Area Under the Curve method using UV-VIS spectroscopy was performed at a wavelength between 350-360 nm. The linearity was obtained at a concentration range of 5-30 µg/ml.  The linear regression equation was Y = 0.1083 x – 0.0310, and the Co-relation Co-efficient was r ² = 0.9996. The sample solution and standard solution are prepared by using water as a diluent. The % RSD was found to be less than 2 in the tablet assay and recovery study. In Method 02, the RP-HPLC method is used. The stationary phase is a C ₁₈ column (250 mm × 4.6 mm, 5 µm), and the mobile phases are a 90:10 mix of methanol and water. The mobile phase flow rate of 1 ml/min, with detection at 237 nm, was used for analytical purposes. Its retention time was 6.48 min. Linearity was obtained at a concentration range of 5–80 µg/ml. The linear regression equation was found to be Y = 49,901.5198 x + 27,678.7201, and the correlation coefficient R² = 0.9999. The %RSD was found to be less than 1.5 in precision, recovery, and robustness studies. The students ‘t’ test values were within the acceptable limit for both the methods. Method 2 is more accurate and highly sensitive in comparison to Method-1.