Phytochemical Screening and Pharmacological Study of Antidiabetic Potential and Bioactive Compounds Present in Allium Sativum

Abstract

Introduction: The present study deals with phytochemical screening and pharmacological study of 
anti-diabetic potential and bioactive compounds present in allium sativum. 
Material and Methods: A study of physicochemical parameters, solubility analysis, and quantitative 
and qualitative analysis of phytochemical screening of allium sativum was done. Then the Antidiabetic activity was studied by alpha-amylase enzyme assay (Starch iodine color assay Method) and 
in-vitro alpha-amylase inhibitory activity (DNSA method). 
Results: Glycoside, Alkaloid, and tannins showed maximum amounts in phytochemical analysis. Invitro alpha-amylase inhibitory activity (DNSA method) shows that the standard drug acarbose 
produces 77.4 % inhibitory activity, and the test sample shows 69.3 % inhibitory activity at a 
concentration of 1250 µg/ml. In-vitro Alpha-amylase inhibitory activity (Starch-Iodine color assay 
method) shows that the standard drug acarbose produces 71.73 % inhibitory activity and the test 
sample shows 81.86 % inhibitory activity at a concentration of 1250 µg/ml.
Conclusions: The present study indicates that allium sativum is effective against a wide range of 
type-2 Diabetes mellitus. In both studies, the percentage of inhibition is nearly similar compared 
with the standard anti-diabetic drug acarbose. So, from the above study, it is concluded that allium 
sativum is effective against a wide range of type-2 Diabetes mellitus.