Formulation of Novel Silver Nanoparticles (Snps) Using Fungal Endophyte Macrosporium Fasciculatum and Evaluation of Their Antimicrobial Potential

Abstract

There is an increasing commercial demand for nanoparticles due to their wide applicability in various areas such as electronics, catalysis, chemistry, energy, and medicine. Metallic nanoparticles are traditionally synthesized by wet chemical techniques, where the chemicals used are quite often toxic and flammable. In this work we have investigated extra cellular biosynthesis of novel silver nanoparticles using fungal extract of recently isolated novel endophytic fungus Macrosporium Fasciculatum. The synthesis process was quite fast and silver nanoparticles were formed within minutes of silver ion coming in contact with the cell filtrate. UV–visible spectrum of the aqueous medium containing silver ion showed a peak at 420 nm corresponding to the plasmon absorbance of silver nanoparticles. Transmission electron microscopy (TEM) micrograph showed formation of well-dispersed silver nanoparticles in the range of 10–30 nm. The process of reduction being extra cellular and fast may lead to the development of an easy bioprocess for synthesis of novel silver nanoparticles. Development of reliable and eco-friendly process for synthesis of metallic nanoparticles is an important step in the field of application of nanotechnology. Further these biologically synthesized nanoparticles were found to be highly toxic against different bacterial and fungal species. The most important outcome of this work will be the development of cost effective, nanoparticles based medicines from Macrosporium Fasciculatumfor the treatment of microbial diseases. This is for the first time that A. alternate fungal extract was used for the synthesis of novel silver nanoparticles.