Comparative Assessment of Opioid Reversal Nasal Spray Efficacy in Countering Fentanyl-Induced Opioid Toxicity in Wistar Albino Rats
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Abstract
Introduction: Anti-opioid nasal spray is already well established in the US market. The opioid reversal activity is characterized by displacement of opioid by anti opioid drug. The formulation in another dosage form studied for its behavioral changes in eperimental animals.
Objectives: The present study investigates a comparative analysis between a commercially available opioid reversal nasal spray and an in-house developed nasal spray, examining their impact on the locomotor activity of Wistar albino rats experiencing Fentanyl overdose.
Methods: The study comprises of 4 groups, first group was labeled as normal control and rest of 3 groups were overdose with fentanyl citrate injection, Group II was kept as an opioid control or negative control group, and rests of two groups Group III and Group IV were administered with marketed and in-house opioid reversal nasal spray respectively. After administration of drug the locomotor activity was measured by using the open field test apparatus. Activity calculated in terms of No of grid cross and freezing.
Results: Statistical data were analysed with a parametric two-way ANOVA followed by Dunnett's multiple comparison tests. The obtained results of test were equivalent as that of marketed formulation.
Conclusions: The comparative evaluation of anti-opioid nasal spray was carried out to treat fentanyl induced opioid overdose, the results of test were equivalent as that of the marketed formulation.