Prevalence of MRSA and Beta-Lactamase-Producing Bacteria in Pus Samples

Abstract

Background: Swift dissemination of bacteria in wound infections and the timely administration of appropriate antibiotics are of utmost importance for healthcare professionals. The aim of this current research was to assess the prevalence and spatial distribution of bacteria obtained from purulent samples, as well as to examine their antibiotic susceptibility patterns among hospitalized patients. Methods: A cross-sectional investigation was conducted over a span of one year. During this period, samples of pus were obtained from the patients. Bacterial isolation and identification were accomplished through culture and biochemical tests, while antibiotic susceptibility testing was performed using the disc diffusion method. Results: S. aureus emerged as the most frequently isolated bacteria. Gram-positive bacteria displayed notable resistance to a range of antibiotics, including Penicillin, Cefoxitin, Ampicillin, Azithromycin, Cotrimoxazole, Cefuroxime, and Cepradine. Remarkably, 58.1% of S. aureus and 100% of S. epidermidis demonstrated resistance to Methicillin, while 51.2% of the isolates were identified as AMPC β-Lactamase producing bacteria. Conclusion: The escalating misuse of antibiotics has led to a surge in Methicillin-resistant, AMPC β-Lactamase, and ESBL-producing bacteria. Our research underscores a concerning trend of rapidly increasing multidrug-resistant (MDR) bacteria, which presents a significant challenge. Consequently, it is imperative to conduct antibiotic susceptibility testing prior to antibiotic administration, and there is an urgent need for ongoing monitoring of antibiotic sensitivity to mitigate the development of resistance.