Nanosuspension of Quercetin and Kaempferol Ameliorates/Attenuates Rifampicin-Isoniazid Induced Hepatocellular Damage in SD Rats

Abstract

Flavonoids are potent antioxidants used for hepatoprotective activity. Quercetin and Kaempferol are widely studied for free radical scavenging activity. The aim of the present study was to develop nanosuspension of quercetin and kaempferol and evaluated its hepatoprotective activity against antitubercular drugs isoniazid and refampicin induced hepatic damage in SD rats. SD rats were used in my research study. In the research study hepatic cell injury was induced due to the administration of isoniazid and rifampicin (100mg/kg) for 21 days in combined form. Quercetin and kaempferol were administered with a dose of 50mg/kg. At the end of the study blood was collected by retro-orbital puncture, and liver was quickly removed under ketamine anesthetics. Quercetin and kaempferol (50mg/kg) caused restoration of biochemical enzymes like AST, ALT, ALP, total bilirubin etc, and also showed normal histology of hepatic tissues. The outcome of the study proved that the nanosuspension of quercetin and kaempferol could potentially to treat hepatocellular damage in SD rats. It’s also has free radical scavenging activity.