Effect of Solvent Concentration of (Tamarindus Indica L.) Ethanol Leave Extract on Secondary Metabolite and Antibacterial Activity Against Staphylococcus Aureus

Abstract

Pimple is a disease the biggest contributor to the problem health skin. Most pimple caused by the bacterium Staphylococcus aureus, seen from prevalence infection skin consequences the bacteria reach 62.8–90.0%. Tamarind leaves (Tamarindus indica L.) in a few study own activity hinder growth of bacteria Because There are potential flavonoids and phenolic compounds as antibacterial. The amount of the content is influenced by the solvent used. The research objective was to determine the effect of variations in solvent concentration on total flavonoid levels, total phenol levels and antibacterial activity against Staphylococcus aureus bacteria from tamarind leaf extract 5%, 10%, 15%, 20%, 25%. Tamarind leaf extract was obtained by maceration using various ethanol concentrations of 50%, 70%, 96%. Followed by the determination of the total levels of flavonoids and phenols using method colorimetry of AlCl₃ and folin ciocalteu in a manner Uv-vis spectrophotometry, while the antibacterial test was carried out using the well method. The results showed that the total levels of flavonoids and phenols at concentrations of 50, 70, 96% is 2.3241; 2.6251; 4.55 mgQE/g and 12.9237; 22.0794; 27.3647 mgGAE/g for phenolic value (p<0.05). Antibacterial testing from concentration extract 5, 10, 15, 20, 25% for 50% solvent, namely 13.28; 14.34; 15.8; 16.61; 18.03 mm, at 70% solvent, namely 13.18; 14.77; 16.71; 17.52; 18.76 mm, whereas in 96% ethanol it is 13.30; 16.08; 17.42; 17.63; 18.94 mm with (p>0.05). The more big concentration solvent the more big total levels of flavonoids, phenols, but not for activity its antibacterial.