Efficacy of Resveratrol and Cisplatin on Biological Activities of Human Tongue Squamous Cell Carcinoma Cell Lines

Abstract

Background: Chemotherapeutic agents as cisplatin (Cis) often showed drug resistance, and various side effects. Thus, the development of novel therapeutic approaches is needed. Resveratrol (RSV) is a natural phytochemical agent with anticarcinogenic activities.

Aim of the study: This study was conducted to investigate the efficacy of RSV with or without different doses of Cis on the biological activities of human tongue squamous cell carcinoma (TSCC) cell lines; namely the viability, migration, and epithelial-mesenchymal transition (EMT) of such tumor cells.

Material and Methods: TSCC cell lines (CAL-27) were used and classified into 6 groups. Intervention agents of RSV with or without Cis at different concentrations were applied. MTT assay and wound healing assay were used to analyze the inhibition of cell proliferation (viability) and the migration of cancer cells, respectively. EMT was investigated by assessment of the immunocytochemical Slug expression.

Results: MTT assay demonstrated that application of RSV or Cis separately inhibited cell viability in a concentration-dependent manner. The combination treatment of RSV and Cis had more cytotoxic effects against cancer cells. Moreover, wound healing assay showed that the combination of RSV with Cis had synergistic inhibition effect on cancer cell migration more than that of single agent treatment. Immunocytochemical assessment showed that combination of RSV with Cis inhibits EMT through reducing Slug expression.

Conclusion: The combination of RSV with Cis could be used as a promising approach for OSCC therapy as they reduced cancer cell viability, migration and inhibited EMT with minimal side effects.