Method Development and Validation of RP-HPLC Method for the Estimation of Sitagliptin Phosphate in Tablet Dosage Form

Abstract

Method Development and Validation of RP-HPLC for the Estimation of Sitagliptin Phosphate in Tablet Dosage Form was developed using Qualisil C18 BDS Column(150×4.5mm,5µ) as stationary phase and Potassium dihydrogen phosphate and Acetonitrile in a ratio 60:40% v/v at pH 4.5 as mobile phase was maintained at a flow rate of 1.0ml/min, the retention time of Sitagliptin Phosphate monohytrate were found to be 2.70min and detection was carried out a 228nm. The significant recovery and minimal coefficients of variation validate the method's appropriateness for concurrent examination of Sitagliptin Phosphate in tablet form. The verified procedure worked well for the tablet's quantitative analysis.