UFLC Method Development and Validation for Anti Retro Viral Drugs: Investigation of Greenness Assessment using Complex GAPI, AGREE and AMGS SPREADSHEET

Abstract

The study's proposal was to establish a method development and validation by green chromatography technique for anti-viral drugs, as well as to assess the proposed method's greenness using various tools, which is one of the emerging developments in the analytical field.. The chromatographic separation is achieved by using Eclipse plusC18(250x4.6, 5μm) column by applying isocratic elution using the mobile phase containing methanol and isopropyl acetate in the ratio of (60:40% v/v) with 1ml/min flow rate. The separation of drugs is achieved by using greener mobile phase.

Results: The retention time of 1.854 min and 8.09 min for Ritonavir and Ombitasvir was found respectively.  The regression co-efficient (R2) is 0.997 for the both drugs. Accuracy & precision is evaluated for the method and found be within the limit and the results were reproducible. Assessment of method was carried out using the three different tools. The proposed method is anticipated to be eco-friendly, alternative to developed method HPLC method in regard to safe solvent, less toxic and less run time. The proposed method was adopted for the estimation of the two samples under study in their combined dosage forms.