The Antimicrobial Efficacy of Different Endodontic Sealers Against Enterococcus Faecalis: An in Vitro Study

Abstract

Objective-This in vitro research compares the effectiveness of four different endodontic sealers against Enterococcus faecalis in terms of antibiotic resistance.

Materials and Methods-Four different endodontic sealers were evaluated for their effectiveness, including resin-based (AH Plus), zinc oxide/eugenol-based (Tubliseal), calcium hydroxide-based (Sealapex), and mineral trioxide aggregate-based (MTA Fillapex). The effectiveness of antibacterial agents employing the agar diffusion technique against E. faecalis. Four wells were created by scraping off agar at evenly spaced spots, filling them with newly mixed root canal sealers, and then inoculating them with E. faecalis. All three plates were left to incubate for 72 hours in an aerobic environment at 37°C. We assessed the diameter of the inhibitory zones after twenty-four, forty- eight, and 72 hours.Unpaired t-test and one-way analysis of variance were used to statistically analyse the data.

Results-The data were statistically analysed using the unpaired t-test and one-way analysis of variance.All of the investigated sealers exhibited some E. faecalis bacterial growth inhibition. These were effective against germs in decreasing order of antibacterial activity: MTA Fillapex>Sealapex> AH Plus >Tubliseal. With longer durations of action, the root canal sealers' effectiveness somewhat declined.

Conclusion-Calcium hydroxide-based sealers had the best antimicrobial effectiveness, followed by resin-based sealers, while MTA-based sealers had the worst.