In Vivo Antioxidant and Diuretic Activity of Anogeissus Latifolia Roxb (Dhava) In Wistar Rat

Abstract

The present study was undertaken to investigate the diuretic activity and acute toxicity profile of the hydroalcoholic extract of the leaves of Anogeissus latifolia (2000Milligram/ kilogram or 3000 Milligram/ kilogram orally).in saline-loaded Wister rat. Wister of either sex was divided into 4 groups (six animals in each group). The control group received normal saline, while the standard group received furosemide (10 mg/kg i.p). Group III to Group VI received the test substances at dose levels of 2000, and 3000 mg/kg orally, respectively. At the end of the fifth hour, urine was collected, and total volume of urine excreted by each animal was recorded. Concentrations of urinary Na⁺ and K⁺ Cl⁻ were determined, and the Na⁺/K⁺ Cl⁻ ratio was calculated to make comparison among the groups. The acute toxicity of the most active fraction was also evaluated. The findings demonstrated that  extract of Anogeissus latifolia  leaves showed significant diuretic (P<0.01), natriuretic (P<0.01), and kaliuretic (P<0.01) effects. At the dose of 3000mg/kg, was show highly diuretic property comparison to2000mg/kg. It also showed a good natriuretic activity.