Invitro Antioxidant Property and Anticancer Property of Different Trihydroxy Flavones- An Experimental Study

Abstract

Classes of naturally occurring substances known as flavonoids are extensively found as secondary metabolites in the plant world are known to possess intriguing therapeutic qualities like anti-inflammatory, anti-allergic, antiviral, antibacterial, and anticancer actions.  Hence this study was conducted to assess the anti cancer and anti oxidant properties of newer compounds like 3,7,3’Trihydroxy flavone, 7,3’,4’Trihydroxy flavone and 6,3’4’Trihydroxy flavone.The study was approved by IEC,  and  conducted in  the department of Pharmacology in Meenakshi medical college Hospital and Research Institute during March  to July 2023. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) was performed to assess the antioxidant and cell viability assay for anticancer properties. The data were presented as Mean ± S.D and the values for IC50 were derived from the plots of the linear regression using SPSS 21 version. To evaluate mean differences at the p < 0.05 as significance, two-way ANOVA was utilised.  Based on the DPPH, ABTS scavenging effect, percentage of inhibition of all the trihydroxy flavones investigated were found to be respectively high compared with the standards. In 24 hours MTT assay compared with control, all three compounds exhibited  statistically significant difference at concentrations of 100, 150, 200, 250 and 300mg/ml and at  50mg/ml concentration the 3, 7, 3’Trihydroxy flavone alone was found to be similar to control.We conclude that all three trihydroxy flavones are effective as anti oxidant and anti cancer agents.