Comparative Analysis of Antibacterial activity of PEG coated and uncoated Biogenic Silver Nanoparticles on Multi-Drug Resistant Pyogenic bacteria isolated from Oral Lesions.

Abstract

The aim of the present study is to evaluate the antibacterial activity of silver nanoparticles that were biogenically synthesized from Klebsiella pneumoniae (BNP) and Neem leaves aqueous extract (NLNPs), followed by Polyethylene glycol capping (PEG). Characterization by UV-Vis spectroscopy, XRD and TEM revealed the topology and morphology of these biosynthesized silver nanoparticles. Average diameter of these nanoparticles was found to be 19.02 nm for BNP and 15 nm for NLNP. These nanoparticles were assessed for their antibacterial activity on the bacterial pathogens that were isolated from oral lesions (S. aureus, E. coli and K. pneumoniae). On the analysis of antibiotic susceptibility, these isolates were found to resist multiple antibiotics and hence they were categorized as MDRs. Antibacterial sensitivity testing by well diffusion method of PEG coated and uncoated BNP and NLNP revealed the strong inhibition potential of these silver nanoparticles on the pyogenic oral isolates S. aureus, E. coli and K. pneumoniae. It can be concluded from this study that coating of the BNP and NLNP by PEG has a poor impact on the antibacterial properties as compared to uncoated BNP and NLNP.