Study of Aerobic Bacteria Isolated from Pus Samples in Tertiary Care Hospital

Abstract

The study aimed to extract and identify aerobic bacteria from pus samples and investigate antibiograms at a rural tertiary care hospital, providing guidance for empirical therapy.

Material & Methods: The study processed pus samples in the Microbiology laboratory, conducted from November 2020 to October 2021 adhering to established standards. Antimicrobial sensitivity testing employed Kirby Bauer's disc diffusion method, following CLSI recommendations.

Results: Prevalent bacteria included Staphylococcus aureus (36.79%), Klebsiella pneumoniae (19.49%), and Escherichia coli (9.09%). Gram-negative bacteria dominated (77%), with common use of Cefoperazone salbactum, Imipenam, Meropenam. Rising concerns involve Methicillin- resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Enterococcus (VRE),    underscoring the importance of antibiotic sensitivity testing.

Conclusion: The study identifies prevalent bacterial isolates, emphasizing Gram-negative dominance and effective antibiotics. Concerns about MRSA and VRE prevalence highlight the need for targeted interventions and antibiotic susceptibility testing, shaping strategies for vulnerable demographics.