Synthesis And Assessment of Antimicrobial Potential of Some Novel 1, 3, 5-Trisubstituted Pyrazolines Derivatives

Abstract

The chalcones and Pyrazoline demonstrate diverse pharmacological profiles, including antimalarial activity with distinct mechanisms of action, making them highly intriguing options.  The synthesis of the intended compounds 3a-3p and 4a-4p was accomplished using the widely recognized Claisen-Schmidt reaction. The resulting products were purified by recrystallization from methanol, resulting in a yield of 60-70%. The required product was synthesized by refluxing chalcone and nicotinic acid hydrazide in n-butanol or methanol, respectively, during the second stage. The synthesized compounds were subjected to physico-chemical characterisation, including investigation of their melting point, FT-IR, 1H-NMR mass spectrum, and elemental properties. The results demonstrated complete concordance between the observed values and the expected values, therefore validating the anticipated structures of the produced molecules. The IR spectra of the synthesized compounds exhibited absorption bands that are indicative of the expected structure of the produced molecules. The NMR spectra of the synthesized compounds exhibited signals corresponding to both aliphatic and aromatic protons, which are indicative of the expected structure of the produced compounds. The synthesized compounds were assessed for their antibacterial activity. The compounds were further assessed for their in vitro antifungal efficacy. The outcomes of the in vitro antibacterial and antifungal activity of the produced compounds (4a – 4p) are exhibited potential antimicrobial and antifungal activity.