Exploring the Immunomodulatory Effects of Leaf Extracts from Parthenium Hysterophorus

Abstract

This study aimed to elucidate the role of immunoglobulins in allergic reactions and investigate the mechanism of IgY in allergic responses by purifying proteins from Parthenium hysterophorus flowers and assessing their allergic activity with IgY. Protein purification methods involved phenol extraction for Parthenium hysterophorus flower proteins and affinity chromatography for IgY purification. The purified allergens were characterized using various techniques including SDS-PAGE, HPLC and IgE-ELISA. SDS-PAGE analysis under reduced conditions identified four major allergenic proteins with molecular weights of 44 kDa, 38 kDa, 18 kDa, and 23 kDa. Cross-reactivity between allergens was assessed using IgY-ELISA at a concentration of 100 ng/ml IgY antibody. These findings suggest that proteins ranging from 18-40 kDa could serve as potential diagnostic markers for patients allergic to Parthenium hysterophorus.