Synthesis Characterization and Antitubercular Evaluation of Thiazolo-Triazole Derivatives Using Microwave Irradiation

Abstract

The present study focuses on the synthesis, characterization, and evaluation of novel thiazolo–triazole derivatives as potential antitubercular agents. A series of compounds (A1–A24) were synthesized using microwave-assisted techniques, offering advantages such as reduced reaction time, improved yields, and environmentally friendly conditions. The synthesized compounds were characterized by standard analytical methods including FT-IR, ¹H NMR, and mass spectrometry to confirm their chemical structures. The antitubercular activity of the synthesized derivatives was evaluated against Mycobacterium tuberculosis H37Rv strain using the Middle brook 7H9 agar method at different concentrations (25, 50, and 100 mcg/ml). The results demonstrated a concentration-dependent activity, with most compounds showing sensitivity at higher concentrations. Among the tested compounds, A16 exhibited the most significant antitubercular activity, while compounds remaining compounds showed moderate activity. Statistical analysis indicated an approximate IC₅₀ value around 85 mcg/ml, suggesting moderate potency of the synthesized derivatives. Overall, the study highlights the potential of thiazolo–triazole hybrids as promising scaffolds for the development of new antitubercular agents, warranting further optimization and pharmacological investigation.