Development and Optimisation of Nanoemulsion as carrier for Cucurbitacin

Abstract

The purpose associated with this study was to create an optimised nanoemulsion formulation    with optimised components wt. % of oil: surfactant-cosurfactant: water, which would be stable, efficient, efficacious and more suitable for delivering the lipophilic Antidiabetic Bioactive Compounds (ABC) via topical application. The optimization was done by using studies over parameters like solubility, stability and emulsification power. Pseudoternary phase diagram was developed for perfect and honed selection of components and further the ratios of selected components was precisely optimised. Organic phase of nanoemulsion was selected as sefsol218 with its maximum solubilising power of drug. Tween80 was selected as surfactant for sefsol218 on basis of area of emulsion on phase diagram, solubility of sefsol218 in surfactant and solubilising power of surfactant to drug CBT. Cosurfactant screened was Transcutol. It was based on nanoemulsion available area (NEAR) on phase diagram with 1:1 Smix ratio of tween80 and six different cosurfactants mixed with sefsol218 and titrated with water. Different components wt. rations were optimized by titrating mixture of sefsol218 and various Smix of tween80 and Transcutol and plotted on Pseudoternary phase diagram to find out the area of nanoemulsion region. 29 formulation were selected and tested for their metastability by thermodynamic cycle testing. Total 11 formulation passed the test and selected as placebo formulations for the study.