In- Vitro Evaluation of Hepatoprotective Potential of Diplocyclos Palmatus (L) C. Jeffrey Leaf Against Paracetamol Induced Liver Toxicity using HEPG2 Cell Line

Abstract

Aim:

To evaluate In-vitro hepatoprotective activity of Hydro alcoholic extract of Diplocyclos palmatus (HADP) leaf extract against paracetamol induced liver toxicity in HepG2 Cell line.

Methods:

HepG2 cells were plated using 96 well  microplate and incubated at 37 °C for 24 h in 5% CO2.After incubation the cells were exposed to 14 Mm paracetamol to induce hepatotoxicity. Cells are then treated with different concentrations of Plant extract (200µg/ml and 400µg/ml and incubated for 24 h in CO2 incubator and silymarin as standard (300µg/ml).  The morphological cells were observed under digital inverted microscope (20X magnification) after 24 h and photographed. The cells were then washed with phosphate-buffer saline (PBS, pH-7.4) and 20 μL of (MTT) solution (5 mg/mL in PBS) was added to each well. The plates were then stand at 37ºC in the dark for 4 h. The formazan crystals were dissolved in 100μL DMSO and the absorbance was read spectrophotometrically at 570 nm.

Results:

The results indicated that paracetamol induced  causes a significant decrease in cell viability about (18.5%).HADP at 200µg &400 µg showed  increase in cell viability (34.1%,70.8%).Silymarin showed increase in cell viability (88.1%).

Conclusion

Our study revealed that HADP leaf extract has potent hepatoprotective effect against paracetamol induced liver toxicity in HepG2 cell line and which may be attributed to decrease in paracetamol induced reactive oxygen species levels and resultant oxidative stress.