Effect of Cryotreated Sodium Hypochlorite on the Fracture Resistance of Endodontically Treated Teeth- An In Vitro Study

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Harpreet Singh Chhabra, Shubhangi, Sanjeev Kumar Srivastava, Kshiti Bhardwaj, Preeti Rastogi, Saraswati Sachan

Abstract

Objective 


To evaluate and compare the impact of cryotreated sodium hypochlorite on the fracture resistance of endodontically treated teeth. 


Methods 


Forty-two extracted human single-rooted mandibular premolars were sectioned horizontally standardized to a root length of 14 mm. Each tooth was wrapped in 0.15 mm aluminum foil, embedded in self-polymerizing resin, and repositioned in light-body silicone impression material. Samples were divided into two groups (n=21) based on irrigation protocol: 


Group A: Sodium hypochlorite at room temperature 


Group B: Cryotreated sodium hypochlorite (2.5°C) 


Chemo-mechanical preparation was performed in both the groups using ProTaper Gold files (F3, 30/.09). Samples were kept at 37°C and 100% humidity before fracture testing using a Universal Testing Machine. A metal indenter applied an increasing load (1 mm/min) until fracture. The maximum load sustained before fracture was recorded and analyzed using the Shapiro-Wilk and Mann-Whitney U tests. 


Results


Group A exhibited significantly higher fracture resistance than Group B, indicating that cryotreated sodium hypochlorite negatively affected dentin strength.


Conclusion


Cryotreated sodium hypochlorite adversely affects dentin’s mechanical properties, leading to reduced fracture resistance. Its clinical use should be approached with caution, and further studies are warranted to determine safe application protocols.

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