Development of A Rapid Test, Optimization and Validation for the Determination of Veterinary Drug Residues in Milk Using Liquid Chromatography Mass-Spectrometry

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Jagdish Kumar Parmar, Shoor Vir Singh, Vikas Gupta, Navaneet Dey

Abstract

Background: Veterinary drugs are antibacterial antibiotics in a broad-spectrum, and these are used to treat animal diseases. Many countries already have abolished the usage of veterinary drugs due to creating residues like nitrofurans, chloramphenicol etc. People already were administered veterinary drugs to increase milk production in animals for more profit and economic lure. This is the reason for the increasing usage of illegal drugs for animals' milk production volume increase and that is practically not useful for consumers. Consumers who deliberately consume the drug-affected animal-derived food products, as many antimicrobial residues are left. It causes serious health diseases, even cancer and allergic diseases among milk Consumers. This calls for a restriction in illegal drug usage for veterinary purposes, Government in India also needs to restrict this drug usage in animals.


Materials and Methods: The identification and confirmation of various veterinary drug residues in milks are available; animal milk products can be scrutinized through various analytical techniques like mass spectrometry and liquid chromatography. All these are sophisticated as well as equipment are widely available these days. The European Commission has described the analytical method validation should be under 2021/808/EC. There are many extraction and instrumental methods for determining antibiotics and veterinary drugs in multiple matrixes but this method includes different categories of compounds in a single run method.


Results: All of the samples along with the standards would be plotted to get a linearity in both matrix match and fortified method. The validation parameters will be determined to justify the genuineness and effectiveness of this method.


Conclusion: The veterinary drug application continuously poses a big challenge in terms of its efficiency, long-term usage and authorisation. There are numerous analytical processes that can be used effectively for protecting consumers’ health in a very less time; more groups of compounds are considered such as sulphonamides, tetracycline and macrolides etc. in a single multi-residual method. All these methods are used under distinct validation parameters and include calibration curve, accuracy, precision, detection limit and quantification. The current study has aimed to obtain multi-residual rapid tests in less time from the milk samples.

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