Molecular Detection of Genes Related to Biofilm Formation in Clinical Pseudomonas Aeruginosa Isolates

Abstract

This study aimed to determine the biofilm formation ability and biofilm association genes (pslD, pelF, fliC, and algD) among P. aeruginosa clinical isolates. Fifty (50) P. aeruginosa isolates were identified out of 250 specimens from burn, wound swabs, urine, blood and sputum. The isolated bacteria were tested for their ability to form biofilm using a microtiter plate method. The expression of (pslD, pelF, fliC, and algD)  genes in biofilm producers isolates was detected using PCR. The MTP detected 100% of isolates as biofilm producers, 32% were strong biofilm producers, 38% were moderate and 30% were weak. The results showed that all isolates have 16SrRNA gene and the investigation of virulence factors associated genes revealed that isolates were harboring algD, pslD, pelF, and fliC gene of the biofilm producers were 96%, 100%, 88% and 58%, respectively.