Document Type : Original Article
Department of Basic Research, National Research Institute for Chemical Technology, Zaria, Nigeria
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Directorate of Research and Development, Nigerian Institute of Leather and Science Technology, Zaria, Nigeria
Department of Biochemistry, School of Basic Medical Sciences, Babcock University, Ilishan-Remo, Nigeria
Petrochemical and Allied Department, National Research Institute for Chemical Technology, Zaria, Nigeria
Paraoxonase and aryl-esterase-1 (PON-1) in serum of rats intoxicated with lead then administered Folic acid and Vitamin-C was investigated in forty male albino rats (160-190 g) randomly separated into 2 groups of 20 rats each namely: Lead acetate (60 mg kg -1) and normal saline was given to each group for 6 weeks and there after administered Folic acid (500 µg kg -1) and/or Vitamin-C (60 mg kg-1) orally for 4 weeks. Blood sample was obtained at the end from each rat for biochemical assessment. Results obtained indicated significant (p<0.05) reduction in activities of arylesterase (60.51±8.52 U L-1) and paraoxonase (74.95±3.63 U L-1) of non-supplemented, rats intoxicated with lead compared to 121.51±7.34 and 98.19±2.25 U L-1 respectively for the control groups. Similarly, the values of catalase (10.54±0.46 U mg-1) and superoxide dismutase (4.93±0.66 U mg-1), differ significantly (p<0.05) in the non-supplemented group intoxicated with lead as compared to 31.62±0.67 U mg-1, and 28.46±1.54 U mg-1 respectively for the control groups. Malonyldialdehyde level (0.58±0.29 nmol L-1) decreased significantly (p<0.05) in the control group as compared to the 5.21±0.16 nmol L-1 observed in the non-supplemented group intoxicated with lead. Significant (p<0.05) decrease was observed for high-density lipoprotein cholesterol level (41.08±0.48 mg L-1) in the non-supplemented, rats intoxicated with lead as compared to the level (78.67±0.66 mg L-1) noticed in the control group. Findings from this study deduced that sub-chronically lead intoxication may cause cardiovascular diseases as evident in the decreased activities of arylesterase and paraoxonase. However, administration of Folic acid and Vitamin-C to the rats intoxicated with lead improves the catalytic activities of PON-1, and this may mitigate lead-induced cardiovascular disease risk.