Short-term Effect of Aerobic Exercise, High-fat Diet and Curcumin Extract on Interleukin-six Gene Expression in Muscle Tissue of Rats

Document Type : Original Article

Authors

Department of Physical Education, Damghan Branch, Islamic Azad University, Damghan, Iran

10.22034/jchr.2021.1931092.1319

Abstract

Interleukin plays six major roles in various types of host defense mechanisms, including immune reactions and acute phase reactions. Therefore, the aim of the present study was to compare the short-term effects of aerobic exercise and high-fat diet and curcumin extract on the expression of interleukin-six gene in muscle tissues in male Wistar rats. For this purpose, 50 two-month-old male rats were prepared and divided into five groups: control group, high-fat diet, curcumin and high-fat diet, exercise and high-fat diet, curcumin + exercise + high-fat diet. Data analysis was compared using analysis test of one-way variance. Bonferroni supplementary test was used to accurately determine the differences between groups. The mean of group differences were also compared using one-sample t-test. The results showed that the mean expression of interleukin gene of 6 subjects showed a significant difference between the 5 groups. The results of Bonferroni post hoc test also showed a significant difference between the control group and other groups. Also, the gene expression of interleukin 6 in the two groups of high-fat diet and high-fat diet + curcumin increased compared to the control group. But in the two groups of high-fat diet + exercise and the group of high-fat diet + exercise + curcumin decreased, which was significant at the error level of 0.05. 

Keywords


1. Kandula V., Kosuru R., Li H., Yan D., Zhu Q., Lian Q., 2016. Forkhead box transcription factor 1: role in the pathogenesis of diabetic cardiomyopathy. Cardiovasculardiabetology. 15(1), 8-15.
2. Dey G., Bharti R., Dhanarajan G., Das S., Dey K.K., Kumar B.P., 2015. Marine lipopeptide Iturin A inhibits Akt mediated GSK3β and FoxO3a signaling and triggers apoptosis in breast cancer. Scientific Reports. 14(3), 5-16.
3. Palomer X., Álvarez-Guardia D., Rodríguez-Calvo R., Coll T., Laguna J.C., Davidson M.M., 2008. TNF-α reduces PGC-1 α expression through NF-κB and p38 MAPK leading to increased glucose oxidation in a human cardiac cell model. Cardiovascular Research. 22(4),703-12.
4. Wang S., Moustaid-Moussa N., Chen L., Mo H., Shastri A., Su R., 2014. Novel insights of dietary polyphenols and obesity. The Journal of Nutritional Biochemistry. 25(1), 1-8.
5. Raoufi A., sirous F., Hoseini A., 2019. The Effect of Eight Weeks of Curcumin Supplementation on the Expression of Some Regulatory Genes of Atrophic Processes in the Heart Tissue of Fatty Adult Fatty Rats. J Fasa Univ Med Sci. 9(2), 1425-1432.
6. Babalwa & Malherbe, Christiaan & Mamushi, Mokadi & Muller, Christo & Joubert, Elizabeth & Louw, Johan & Pheiffer, Carmen. 2019. Adipose tissue as a possible therapeutic target for polyphenols: A case for Cyclopia extracts as anti-obesity nutraceuticals. Biomedicine pharmacotherapy. 39(1),83-98.
7.  Shailendra P., Singh S.P., Bellner L., Vanella L., Cao J., Falck J.R., Kappas A., 2016. Downregulation of PGC-1α prevents the beneficial effect of EET-heme oxygenase-1 on mitochondrial integrity and associated metabolic function in obese mice. Journal of Nutrition and Metabolism. 33(3), 42-59.
8. Lundman P., Boquist S., Samnegård A., Bennermo M., Held C., Ericsson C.G., Silveira A., Hamsten A., Tornvall P., 2007. A high-fat meal is accompanied by increased plasma interleukin-6 concentrations. Nutr Metab Cardiovasc Dis. 17(3), 195-202.
9. Müller S., Martin S., Koenig W., Hanifi Moghaddam P., Rathmann W., Haastert B., Giani G., Illig T., Thorand B., Kolb H., 2002. Impaired glucose tolerance is associated with increased serumoncentrations of interleukin 6 and co-regulated acute-phase proteins but not TNF-alpha or its receptors. Diabetologia.  45(6), 805-12.
10. Kim J.H., Bachmann R.A., Chen J., 2009. Interleukin-6x and insulin resistance. Vitam Horm.  80(3), 613-33.
11. Huang W.C., Chiu W.C., Chuang H.L., Tang D.W., Lee Z.M., Wei L., 2015. Effect of Curcumin S upplementation
on Physiological Fatigue and Physical P erformance in Mice. Nutrients. 7(2), 905- 921.