Role of A2-adrenoceptors in Rat Heart with the Model of Myocardial Infarction

Document Type : Original Article


1 Associate Professor (Department of Human Health Protection) Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia

2 Рostgraduate (Department of Human Health Protection) Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia

3 Senior Lecturer (Department of Human Health Protection) Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia

4 Professor (Department of Human Health Protection) Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia


Chronic adrenergic and angiotensin-energic stimulation of the heart muscle is one of the main causes of the onset and development of heart failure. α2-AR is widespread in the cardiovascular system and can be further applied and introduced into the heart's regenerative cell therapy. This study's objective was to study the effect of α2-AR stimulation on the performance of an isolated heart in rats with a model of myocardial infarction. To study the mechanisms of MI, experiments were carried out according to the classical technique of H. Selye - ligation of the anterior branch of the left coronary artery and perfusion of a Langendorff heart with a model of myocardial infarction. We studied the parameters of myocardial activity by changes in heart rate, as well as the left ventricle pressure (LVP), and the CF (dp/dtmax)/(dp/dtmin) of the left ventricle. Coronary dilator activity was assessed by measuring the outflow of the perfusion solution through the coronary arteries. The amplitude of the left ventricle's pressure wave after application of the α2-AR agonist clonidine hydrochloride at a concentration of 10-6 M to the perfused solution increased by 44% (p≤0.05) from the initial values. The rate of contraction and the rate of relaxation of the left ventricular myocardium also increased by 20% (p≤0.05) and 37% (p≤0.05), respectively. The activation of α2-AR in adult rats' isolated hearts with a model of myocardial infarction caused a decrease in heart rate by 18% (p≤0.05) and CF by 2% (p≤0.05).


1. sangy S., Bahaoddini A., Tavassoli A., 2020. Evaluation of Histopathological Changes of Osteoarthritis on the Cardiovascular System by Gavage of Hydro Alcoholic Extract of Wild Anemone (Papaver Rhoeas L.) in Male Rats. Progress in Chemical and Biochemical Research. 3(4), 402-411.
2. Ukwubile C.A., Ikpefan E.O., Bingari M.S., Tam L., 2019. Acute and subchronic toxicity profiles of Melastomastrum capitatum (Vahl) Fern. (Melastomataceae) root aqueous extract in Swiss albino mice. Progress in Chemical and Biochemical Research. 2(2), 74-83.
3. Khan Muluh E., Odokpe Ugbede A., Tor-Anyin T.A., 2019. Screening of Cassia Sieberiana (Fabaceae) Leaf Extract for In-Vitro Anti-Microbial and Anti-Ulcer Activities. Progress in Chemical and Biochemical Research. 2(3), 143-149.
4. Sangy S., Bahaoddini A., Alsadat Miryousefiata F., 2020. Therapeutic Effects of Hydro-alcoholic Extract of Papaver Rhoeas on Cardiovascular side effect of MIA Induced Osteoarthritis in male Rat. Progress in Chemical and Biochemical Research. 3(4), 340-349.
5. Hassan Zahraee A., 2019. Manufacturing High Quality Medical Catheters: A Technology Mastered by Few. Advanced Journal of Chemistry-Section B. 1(1), 1-2.
6. Lorenz W.,1990. Expression of three alpha 2-adrenergic receptor subtypes in rat tissues: implications for alpha 2 receptor classification. Mol Pharmacol. 38, 599-603.
7. Berkowitz D.E.,1994. Localization of messenger RNA for three distinct alpha 2-adrenergic receptor subtypes in human tissues: Evidence for species heterogeneity and implications for human pharmacology. Anesthesiology. 81, 1235–44.
8. Mariappan R., 2014.Comparing the effects of oral clonidine premedication with intraoperative dexmedetomidine infusion on anesthetic requirement and recovery from anesthesia in patients undergoing major spine surgery. Neurosurg Anesthesiol. 26(3), 192–197.
9. Nahrendorf M.,2001. Serial cine-magnetic resonance imaging of left ventricular remodeling after myocardial infarction in rats. J Magn Reson Imaging. 14(5), 547-555.
10. Gilsbach R., 2010. Sympathetic alpha2-adrenoceptors prevent cardiac hypertrophy and fibrosis in mice at baseline but not after chronic pressure overload. Cardiovasc Res. 86(3), 432–442.
11. Zefirov T.L., 2011. Comparative Analysis of the Impact of α1- and α2-Adrenoreceptor Blockade on Cardiac Function in Rats during Postnatal Ontogeny. Bul Exp Biol Med. 151(6), 664-666.
12. Ziyatdinova N.I., 2018. Effect of α2-Adrenoceptor Stimulation on Functional Parameters of Langendorff-Isolated Rat Heart. Bul Exp Biol Med. 165(5), 593-596.
13. Kuptsova A.M., 2016. Yohimbine influence on myocardium contractile activity among newborn rats. Int J of Adv Biotech Res. 7(4), 1305-1309.
14. Gilsbach R., 2012. Are the pharmacology and physiology of α2-adrenoceptors determined by α2-heteroreceptors and autoreceptors respectively? Br J Pharmacol. 165(1), 90-102.
15. Arekhi M., & Jamshidi M. 2018. Influences of inorganic binder on photocatalytic oxidation (PCO) and degradation of nano/micro TiO2 containing acrylic composites. Progress in Organic Coatings. 115, 1-8.
16. Korotaeva Iu V., 2015. Alpha2-adrenergic receptors of the myocardium (Literature review). News of the Komi Scientific Center of the Ural Branch of the Russian Academy of Sciences. 2(22), 57-64.
17. Zefirov T.L.,2014. Effect of α2-Adrenoceptor Stimulation on Cardiac Activity in Rats. BulExp Biol Med. 157(2), 194-197.
18. Pimenov O.I., 2019. Myocardial α2-adrenergic receptors prevent cardiac hypertrophy under increased angiotensin II. Collection: Receptors and intracellular signaling, ed. V.P. Zinchenko, A.V. Berezhnova. 268-273.
19. Arekhi M., Terry L. G., John G.F., Al-Khayat J. A., Castillo A.B., Vethamony P., & Clement T.P. 2020. Field and laboratory investigation of tarmat deposits found on Ras Rakan Island and northern beaches of Qatar. Science of The Total Environment. 735, 139516.
20. Dzidzhikhiia K.M.,2012. Obtaining a model of large-focal myocardial infarction in an acute experiment on rats. Fundamental Research. 4, 270-273.
21. Ershov K.I.,2014. Modification of the myocardial ischemia/reperfusion method. Medicine and Education in Siberia. 3, 62.
22. Selyt H.,1960. Simple techniques for the surgical occlusion of coronary vessels in the rat. Angiology. 11, 398-408.
23. Hosseinpour J., & Mahdavy-Moghaddam H., 2020. Computational study of magnetic field effects on the nozzle of hydrogen micro flame. Combustion and Flame. 220, 247-256.
Volume 11, Issue 2
May 2021
Pages 129-134
  • Receive Date: 25 October 2020
  • Revise Date: 23 May 2021
  • Accept Date: 28 December 2020
  • First Publish Date: 01 May 2021