Reaction of Maize Varieties to Aspergillus flavus and Aflatoxin Production

Document Type : Original Article


1 Department of Plant Pathology, Damghan branch, Islamic Azad University, Damghan, Iran

2 Plant Protection Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, AREEO, Sari, Iran

3 Food safety Research Center (salt), Semnan University of Medical Sciences, Semnan, Iran

4 Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Center, AREEO, Yazd, Iran

5 Plant Protection Research Department, Semnan (Shahrood) Agricultural and Natural Resources Research and Education Center, AREEO, Shahrood, Iran



Aflatoxins, are very toxic mixture and having the potential to cause cancer, produce by some the fungi, mostly Aspergillus flavus and Aspergillus parasiticus, during their growth, harvest and storage on crops such as wheat, corn, cottonseed, peanut, pistachio, etc. For appraising the reaction of maize varieties to A. flavus growth and produced aflatoxin, five varieties of maize, namely KSC400, KSC403, KSC600, KSC703, KSC704, were selected. An isolate A. flavus that produce afalatoxin was used for inoculation of maize kernels (as in vitro conditions). In a statistic completely random design with three replications, 10 gr of kernels of selected cultivars were inoculated with 1×106 spores/ml of fungal spore suspension. After eight days, the percentage of fungal growth and the colonization of maize kernels were calculated. The aflatoxin B1 produced in contaminated all maize cultivars were measured with high performance liquid chromatography (HPLC). Statistical analyses showed a significant difference (α=0.05) between the fungal growth percentages and the amount of aflatoxin B1 in the kernels of tested maize cultivars. Also, the results showed that among mentioned varieties, KSC600 was the least susceptible variety to the growth of Aspergillus. The amount of produced aflatoxin B1 was variable among the varieties. KSC600 had the least whereas KSC403 had the highest rate of aflatoxin B1 content.


1. FAO/WHO Expert Committee.  2017. Evaluation of certain contaminants in food (Eighty-third report of the Joint FAO/WHO Expert Committee on Food Additives). WHO Technical Report Series, No. 1002.
2. FAO. 2004. Worldwide regulations for mycotoxins in food and feed in 2003. FAO
3. Probst C., Schulthess F., Cotty P., 2010. Impact of Aspergillus section Flavi community structure on the development of lethal levels of aflatoxins in Kenyan maize (Zea mays). Journal of Applied Microbiology. 108(2), 600-610.
4. Turner P.C., Moore S.E., Hall A.J., Prentice A.M., Wild C.P., 2003. Modification of immune function through exposure to dietary aflatoxin in Gambian children. Environmental Health Perspectives. 111(2), 217.
5. Gong Y., Hounsa A., Egal S., Turner P.C., Sutcliffe A.E., Hall A.J., Cardwell K., Wild C.P., 2004. Postweaning exposure to aflatoxin results in impaired child growth: a longitudinal study in Benin, West Africa. Environmental Health Perspectives. 1334-1338.
6. Wilson D.M., Payne G.A. 1994. Factors affecting Aspergillus flavus group infection and aflatoxin contamination of the crops. In: The Toxicology of Aflatoxins: Human Health, Veterinary, and Agricultural Significance (Eaton DL, Groopman JD, eds). San Diego, CA:Academic Press, 309–325.
7. Van Egmond H., 1995. Mycotoxins: regulations, quality assurance and reference materials. Food Additives & Contaminants. 12(3), 321-330.
8. Moghaddam M., Goltapeh E., Hokmabadi H., Haghdel M., Mortazavi A., 2006. Evaluation of susceptibility of pistachio cultivars to aflatoxigenic Aspergillus flavus and aflatoxin B1 production. Acta Horticulturae. 726, 655-658.
9. Richard J., Payne G., eds, Desjardins A., Maragos C., Norred W., Pestka J., 2003. Mycotoxins: risks in plant, animal and human systems. CAST Task Force Report. 139, 101-103.
10. Wu F., 2004. Mycotoxin risk assessment for the purpose of setting international regulatory standards. Environmental Science & Technology. 38(15), 4049-4055.
11. Bhatnagar D., Rajasekaran K., Cary J.W., Brown R., Yu J., Cleveland T.E., 2008. Molecular approaches to development of resistance to preharvest aflatoxin contamination. Mycotoxins: Detection Methods, Management, Public Health and Agricultural Trade. 257-276.
12. Cleveland T.E., Dowd P.F., Desjardins A.E., Bhatnagar D., Cotty P.J., 2003. United States Department of Agriculture—Agricultural Research Service research on pre‐harvest prevention of mycotoxins and mycotoxigenic fungi in US crops. Pest Manag Sci. 59(6‐7), 629-642.
13. Brown R., Chen Z., Menkir A., Cleveland T., 2006. Proteomics to identify resistance factors in corn-a review. Mycotoxin Research. 22(1), 22-26.
14. Brown R., Chen Z., Gembeh S., Cleveland T., Bhatnagar D., Howard K., 2004. Identification of natural resistance in corn against mycotoxin-producing fungi. Rec Adv Food Sci. 4, 85-96.
15. Brown R.L., Chen Z.Y., Warburton M., Luo M., Menkir A., Fakhoury A., Bhatnagar D., 2010. Discovery and characterization of proteins associated with aflatoxin-resistance: evaluating their potential as breeding markers. Toxins. 2(4), 919-933.
16. Campbell K., White D., 1995. Evaluation of corn genotypes for resistance to Aspergillus ear rot, kernel infection, and aflatoxin production. Plant Disease. 79(10), 1039-1045.
17. Menkir A., Brown R.L., Bandyopadhyay R., Cleveland T.E., 2008. Registration of six tropical maize germplasm lines with resistance to aflatoxin contamination. Journal of Plant Registrations. 2(3), 246-250.
18. CHEN Z.Y., Brown R.L., Damann K.E., Cleveland T.E., 2010. PR10 expression in maize and its effect on host resistance against Aspergillus flavus infection and aflatoxin production. Molecular Plant Pathology. 11(1), 69-81.
19. Chen Z.Y., Brown R., Damann K., Cleveland T., 2007. Identification of maize kernel endosperm proteins associated with resistance to aflatoxin contamination by Aspergillus flavus. Phytopathology. 97(9), 1094-1103.
20. Chen Z.Y., Brown R., Rajasekaran K., Damann K., Cleveland T., 2006. Identification of a maize kernel pathogenesis-related protein and evidence for its involvement in resistance to Aspergillus flavus infection and aflatoxin production. Phytopathology. 96(1), 87-95.
21. Cary J., Rajasekaran K., Yu J., Brown R., Bhatnagar D., Cleveland T., 2009. Transgenic approaches for pre-harvest control of mycotoxin contamination in crop plants. World Mycotoxin Journal. 2(2), 203-214.
22. Rajasekaran K., Jaynes J., Cary J., 2009.American Chemical Society: Washington, DC, USA. pp. 119-142.
23. Rajasekaran K., Cary J., Cleveland T., 2006. Prevention of preharvest aflatoxin contamination through genetic engineering of crops. Mycotoxin Research. 22(2), 118-124.
24. Brown R.L., Chen Z.Y., Cleveland T.E., Menkir A., Fakhoury A., 2009. American Chemical Society: Washington, DC. pp. 157-165.
25. Allameh A., Abyaneh M.R., Shams M.R., Rezaee M., Jaimand K., 2002. Effects of neem leaf extract on production of aflatoxins and activities of fatty acid synthetase, isocitrate dehydrogenase and glutathione S-transferase in Aspergillus parasiticus. Mycopathologia. 154(2), 79-84.
26. Gourama H., Bullerman L.B., 1995. Aspergillus flavus and Aspergillus parasiticus: Aflatoxigenic fungi of concern in foods and feeds: A review. Journal of Food Protection®. 58(12), 1395-1404.
27. Moghadam M.M., Hokmabadi H., 2010. Study on the Effect of Pistachio Testa on the Reduction of'Aspergillus flavus' Growth and Aflatoxin B1 Production in Kernels of Different Pistachio Cultivars. Australian Journal of Crop Science. 4(9), 744-749.
28. Payne G.A., 1998. Process of contamination by aflatoxin-producing fungi and their impact on crops. In: Mycotoxins in Agriculture and Food Safety. K.K. Sinha and D. Bhatnagar (eds.), Marcel Decker Inc, New York. pp. 279-300.
29. Cole R., Hill R., Blankenship P., Sanders T., Garren K., 1982. Influence of irrigation and drought stress on invasion by Aspergillus flavus of corn kernels and peanut pods. Developments in Industrial Microbiology. 23, 229–236.
30. Diener U.L., Cole R.J., Sanders T., Payne G.A., Lee L.S., Klich M.A., 1987. Epidemiology of aflatoxin formation by aspergillus flavus. Annual Review of Phytopathology. 25(1), 249-270.
31. Łata B., Tomala K., 2007. Apple peel as a contributor to whole fruit quantity of potentially healthful bioactive compounds. Cultivar and year implication. Journal of Agricultural and Food Chemistry. 55(26), 10795-10802.
32. Wallin J.R. 1986. Production of aflatoxin in wounded and whole maize kernels by Aspergillus flavus. Plant disease. 70, 429-430.
33. Zuber M.S. 1977. Influence of plant genetics on toxin production in corn. Proceeding of Conference on Mycotoxins in Human and Animal Health, College Park, Md. USA. p. 173-179.
34. Amalraj, S.F.A. and Meshram M.K. 1981. Role of certain morphological characters in relation to boll rot resistance in Gossypium hirsutem L.Turnalba 31:381–383.
35. Mehan V.K., 1989. Screening groundnuts for resistance to seed invasion by Aspergillus flavus and to aflatoxin production. In: McDonald D., Mahan V.K. and Hall S.D. (eds.), Aflatoxin contamination of groundnut: Proceedings of the International Workshop. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, pp. 323-334.
36. Ghewande M., Nagaraj G., Jhala R., Basu M., Reddy P., 1986. Dry seed resistance to Aspergillus flavus colonisation and aflatoxin production in groundnut. Indian J Plant Prot. 14, 59-64.
37. Gradziel T.M., Wang D., 1994. Susceptibility of California almond cultivars to aflatoxigenic Aspergillus flavus. HortScience. 29(1), 33-35.
38. Brown R.L., Cotty P.J., Cleveland T.E., Widstrom N.W., 1993. Living maize embryo influences accumulation of aflatoxin in maize kernels. Journal of Food Protection®. 56(11), 967-971.
39. Brown R., Cleveland T., Payne G., Woloshuk C., Campbell K., White D., 1995. Determination of resistance to aflatoxin production in maize kernels and detection of fungal colonization using an Aspergillus flavus transformant expressing Escherichia coli b-glucuronidase. Phytopathology. 85(9), 983-989.
40. Guo B.Z., Russin J.S., Cleveland T.E., Brown R.L., Widstrom N.W., 1995. Wax and Cutin Layers in Maize Kernels Associated with Resistance to Aflatoxin Production by Aspergillus ffavus. Journal of Food Protection®. 58(3), 296-300.
41. Gembeh S.V., Brown R.L., Grimm C., Cleveland T.E., 2001. Identification of chemical components of corn kernel pericarp wax associated with resistance to Aspergillus flavus infection and aflatoxin production. Journal of Agricultural and Food Chemistry. 49 (10), 4635-4641.
42. Russin J., Guo B., Tubajika K., Brown R., Cleveland T., Widstrom N., 1997. Comparison of kernel wax from corn genotypes resistant or susceptible to Aspergillus flavus. Phytopathology. 87(5), 529-533.
43. Brown R.L., Chen Z.Y., Menkir A., Cleveland T.E., Cardwell K., Kling J., White D.G., 2001. Resistance to aflatoxin accumulation in kernels of maize inbreds selected for ear rot resistance in West and Central Africa. Journal of Food Protection. 64(3), 396-400.
44. Menkir A., Brown R.L., Bandyopadhyay R., Chen Z.Y, Cleveland T.E., 2006. A USA–Africa collaborative strategy for identifying, characterizing, and developing maize germplasm with resistance to aflatoxin contamination. Mycopathologia. 162 (3), 225-232.