Evaluation of Oxidative Stress Biomarkers in Patients with Breast cancer Compared with Healthy Subjects

Document Type : Original Article


1 Department of Laboratory Sciences, Zahedan Branch, Islamic Azad University, Zahedan, Iran

2 Health Clinical Sciences Research Center, Zahedan Branch, Islamic Azad University, Zahedan, Iran

3 Department of Midwifery, Faculty of Medical Science, Zahedan Branch, Islamic Azad University, Zahedan, Iran

4 Department of Nursing, Zahedan Branch, Islamic Azad University, Zahedan, Iran


Breast cancer is one of the most common malignancies in women with the highest mortality rate in women. Oxidative stress characteristics play a large role in the risk factor of tumors. In this study, we measure the oxidative stress indices of malondialdehyde(MDA), Total antioxidant capacity(TAC) in women with breast cancer compared to healthy subjects.This case-control study was performed on 30 women with a definitive diagnosis of breast cancer and 30 healthy women. The subjects were matched in terms of age and sex and then blood samples were taken from them and collected in vials EDTA .Oxidative stress was measured by spectrophotometry. MDA level was significantly higher in cases (2.48± 0.73 ) compared to controls (1.4± 0.63 (. In contrast, there were lower TAC levels in cases compared to controls and was statistically significant . The data showed  that higher MDA levels in BC patients than in the control group. The results suggest that increased serum MDA and decreased TCA activity may be due to oxidative stress, which may play an important role in BC formation.


1. Matés J.M., Sánchez-Jiménez F.M., 2000 . Role of reactive oxygen species in apoptosis: implications for cancer therapy. The Int J Biochem Cell Biol .32,157-70.
 2. MatÉs J.M., Pérez-Gómez C., De Castro N., 1999. Antioxidant enzymes and human diseases. Clin Biochem. 32, 595-603.
 3. Hou M.F., Lin S.B., Yuan S.S.F., 2003. The clinical significance between activation of nuclear factor kappa B transcription factor and overexpression of HER-2/neu oncoprotein in Taiwanese patients with breast cancer. Clin Chimi Acta . 334, 137-44.
 4. Ahmedin Jemal D., Tiwari R.C., Murray T., 2004. Cancer statistics. CA Cancer J Clin. 8-29.
 5. Thangaraju M., Vijayalakshimi T., Sachdanandam P.,1994. Effect of tamoxifen on lipid peroxide and antioxidative system in postmenopausal women with breast cancer. Cancer . 74,78-82.
 6. Punnonen K., Ahotupa M., Asaishi K., Hyoty M., Kudo R., Punnonen R.,1994. Antioxidant enzyme activities andoxidative stress in human breast cancer. J Cancer Res Clin Oncol. 120, 374-7.
 7. Portakal O., Ozkaya O., Erden Inal M., 2000. Coenzyme Q10 concentrations and antioxidant status in tissues of breast cancer patients. Clin Biochem. 33, 279-84.
 8. Iscan M., Coban T., Cok I., 2002. The organochlorine pesticide residues and antioxidant enzyme activities in human breast tumors: is there any association? Breast Cancer Res Treat. 72, 173-82.
 9. Kumaraguruparan R., Subapriya R., Viswanathan P, Nagini S., 2002. Tissue lipid peroxidation and antioxidant status in patients with adenocarcinoma of the breast. Clinica Chim Acta. 325,165-70.
 10. Kumaraguruparan R., Subapriya R., Kabalimoorthy J., Nagini S., 2002. Antioxidant profile in the circulation of patients with fibroadenoma and adenocarcinoma of the breast. Clin Biochem . 35, 27-9
11. Rashich F., Klaunig J.E., Xu Y., Isenberg J.S., 1998. The role of oxidative stress in chemical carcinogenesis. Environ Health Perspect . 106, 289-95.
 12. Kilic j., Bandyopadhyay U., Das D., Banerjee R.K., 1999. Reactive oxygen species: oxidative damage and pathogenesis. Current Science . 77, 65-88.
 13.Chandramathi D., Vaca C.E., Wilhelm J., Harms-Ringdahl M., 1988. Interaction of lipid peroxidation products with DNA. A Review. Mutat Res. 195,137-49.
 14.Halvorsen B., Ashokkumar T., 2004. Antioxidants: new-generation therapeutic base for treatment of polygenic disorders. Current Science . 54, 133-87.
 15.Uraj D.L., Austin J.H., 1932. Spectrophotometric studies I. Spectrophotometric constants for common hemoglobin derivatives in human, dog, and rabbit blood. J Biol Chem. 98,719-33.
 16. Yagi K., 1987. Lipid peroxides and human diseases. Chem Phy Lipids. 45, 337-51.
 17. Kakkar P., Das B., Viswanathan P., 1984. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys. 21,130-2.
 18. Sinha A.K., 1972 . Colorimetric assay of catalase. Anal Biochem . 47, 389-94.
 19. Sullivan M.X., 1955. Estimation of ascorbic acid. J Assoc of Agric Chem. 38, 54-9.
 20. Bieri J.G., Teets I., Belavady B., Andrews E.L., 1964  . Serum vitamin E levels in a normal adult population in Washington, DC, area. Exp Biol Med. 117, 131-3.
 21. Smith J.C., Butrimovitz G.P., Purdy W.C., 1979. Direct measurement of zinc in plasma by atomic absorption spectroscopy. Clin Chem . 25,1487-91.
 22. Pisani P., Parkin D., Ferlay J., 1993. Estimates of the worldwide mortality from eighteen major cancers in 1985. Implications for prevention and projections of future burden. Int J of Cancer. 55, 891-903.
 23. Huang M.J., 2018. Oxidative stress status and cancer: Methodology applicable for human studies. Free Rad Biol Med. 10,175-6.
 24. Halliwell B., Gutteridge J.M., 1999. Free radicals in biology and medicine. Oxford University Press. 15,145-6.
 25. Huang Y.L., Sheu J.Y., Lin T.H., 1999. Association between oxidative stress and changes of trace elements in patients with breast cancer. Clin Biochem. 32, 131-6.
 26. Khanzode S.S., Muddeshwar M., Khanzode S.D., Dakhale G.N., 2004 . Antioxidant enzymes and lipid peroxidation in different stages of breast cancer. Free Radic Res. 38, 81-5.