Examining the Existence of Synthetic Dyes in the Nuts Offered in Marivan County, West of Iran

Document Type : Original Article


1 Master’s Student, Department of Health and Food Safety, Faculty of Health, Qazvin University of Medical Sciences, Qazvin, Iran

2 Associate professor, Health Products Safety Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

3 Associate Professor, Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

4 Food Quality Control Laboratory, Food and Drug Administration, Qazvin University of Medical Sciences, Qazvin, Iran


These days, making use of synthetic dyes in producing and processing food, such as nuts, is enhancing due to customer attention. Considering that the application of this type of dyes is not permitted in accordance with the current regulations of the country, controlling nut products in terms of dye is required and overriding. In this study, sampling was performed randomly in 10 nut distribution centers at one-week intervals. Samples were tested respecting the type of dye. A Thin-Layer Chromatography (TLC) method was applied to identify the types of dye. After conducting tests on different samples of nuts, it was recognized that different synthetic dyes such as tartrazine, quinoline yellow, and Ponceau 4R were used in the sample nuts. Of the 50 samples tested and analyzed, 23 samples (46%) had non-permitted synthetic dye, and 27 samples (54%) had permitted synthetic dye. The frequency distribution of synthetic dye among different nuts was significantly different. Furthermore, consumption of almond is associated with lower risk of permitted and non-permitted synthetic compared to pistachio (p < 0.05). Application of these types of dye, due to their glamorous appearance, will be significantly improved in the future. Therefore, with regard to the high consumption of synthetic dyes in food and their adverse effects on health, it seems that measures like increasing the level of awareness of producers and consumers about the effects of consumption or non-consumption of these compounds as well as continuous monitoring of units by health inspectors are necessary. 


1.Batada A., Jacobson M.F., 2016. Prevalence of Artificial Food Colors in Grocery Store Products Marketed to Children. Clin Pediatr. 55(12), 1113-1119.
2.Stich E., 2016. Food Color and Coloring Food: Quality, Differentiation and Regulatory Requirements in the European Union and the United States. Handbook on Natural Pigments in Food and Beverages: Elsevier. 3-27.
3.Oplatowska-Stachowiak M., Elliott C.T., 2017. Food Colors: Existing and Emerging Food Safety Concerns. Crit Rev Food Sci Nutr. 57(3), 524-548.
4.Arnold L.E., Lofthouse N., Hurt E., 2012. Artificial Food Colors and Attention-Deficit/Hyperactivity Symptoms: Conclusions to Dye for. Neurotherapeutics. 9(3), 599-609.
5.Al-Kharosi A.S., Al-Sabari W.A., AL-shidhani F.H., Alfarsi S.A., Taha S.M., 2020. A Simple Method for Simultaneous Determination of Commonly Used Artificial Food Colors and Preservatives in Soda, Jam, and Yogurt by HPLC-PDA. Braz J Anal Chem. 7.
6.Rao P., Sudershan R., 2008. Risk Assessment of Synthetic Food Colors: a Case Study in Hyderabad, India. Int J Food Saf Nutr Publ Health. 1(1), 68-87.
7.Saleem N., Umar Z.N., 2013. Survey on the Use of Synthetic Food Colors in Food Samples Procured from Different Educational Institutes of Karachi city. J Trop Life Sci. 3(1), 1-7.
8.Leo L., Loong C., Ho X.L., Raman M.F.B., Suan M.Y.T., Loke W.M., 2018. Occurrence of Azo Food Dyes and Their Effects on Cellular Inflammatory Responses. Nutrition. 46, 36-40.
9.Revision C., Tomes F.X., Collins C., Robert L., Sprado M., Shackelford D., 2002. Proposed Testing Guidelines for Developmental Toxicity Studies. Regul Toxicol Pharmacol. 30, 39-44.
10.Weiss B., 2012. Synthetic Food Colors and Neurobehavioral Hazards: the View from Environmental Health Research. Environ Health Perspect. 120(1), 1-5.
11.Nigg J.T., Lewis K., Edinger T., Falk M., 2012. Meta-analysis of Attention-Deficit/Hyperactivity Disorder or Attention-Deficit/Hyperactivity Disorder Symptoms, Restriction Diet, and Synthetic Food Color Additives. J Am Acad Child Psy. 51(1), 86-97.
12.Dilrukshi P.T., Munasinghe H., Silva A.B.G., De Silva P.G.S.M., 2019. Identification of Synthetic Food Colours in Selected Confectioneries and Beverages in Jaffna District, Sri Lanka. J Food Qual. 2019.
13.Kris-Etherton P.M., Hu F.B., Ros E., Sabaté J., 2008. The Role of Tree Nuts and Peanuts in the Prevention of Coronary Heart Disease: Multiple Potential Mechanisms. J Nutr. 138(9), 1746S-1751S.
14.ISIRI., 1997. Standard Methods for Preparation of Food Samples and Enumeration of Microorganisms in Food.
15.Heshmati A., Hakim S.S., Safari A.A., Afshar A., Amini K., Rabi N., Nasiri E., 2014. Survey of Occurrence and Type of Artificial Colors in Nuts and Conventional Ice Cream Supplied in Karaj City in 2012. Alborz University of Medical Sciences J. 3(3), 165-170.
16.NIS. Permitted Artificial Colors Persian. ed t, editor: Food and Drug Administration; 2002.
17.Ashok V., Agrawal N., Esteve-Romero J., Bose D., Dubey N.P., 2017. Detection of Methyl Orange in Saffron and Other Edibles Using Direct Injection Micellar Liquid Chromatography. Food Anal Methods. 10(1), 269-276.
18.Rezaei M., Safar Abadi F., Sharifi Z., Karimi F., Alimohammadi M., Susan Abadi R.A., Roostaei H., 2015. Assessment of Synthetic Dyes in Foodstuffs Produced in Confectioneries and Restaurants in Arak, Iran. Thrita. 4(1) e22776.
19.Soltan Dallal M.M., Vahedi S., Najjarian A., Dastbaze A., Kaffashi T., Pirhadi E., Kamkar A., Faramarzi T., Mahdavi V., 2008. Study of Concentration of Colors Added to Juice of Black Cherry and Juice of Barberry on Display in Shop in City of Tehran. Journal of Payavard Salamat. 2(1), 55-62.
20.Farzianpour F., Khaniki G.J., Younesian M., Ghahferkhi B.B., Sadeghi M., Hosseini S., 2013. Evaluation of Food Color Consumption and Determining Color Type by Thin Layer Chromatography. Am J Appl Sci. 10(2), 172.
21.Hinton D.M., 2000. US FDA "Redbook II" Immunotoxicity Testing Guidelines and Research in Immunotoxicity Evaluations of Food Chemicals and New Food Proteins. Toxicol Pathol. 28, 467-478.
Volume 11, Issue 2
May 2021
Pages 181-188
  • Receive Date: 30 June 2020
  • Revise Date: 08 January 2021
  • Accept Date: 15 March 2021
  • First Publish Date: 23 March 2021