Evaluation of Physicochemical Methods for Dormancy Breakage and Germination of Datura stramonium Seeds

Document Type: Original Article


1 Nutrition and Dietetics Program, School of Health Sciences, Universiti Sains Malaysia Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia

2 Faculty of Basic Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran


Datura stramonium L. broadly performed in plant virology study has a significant seed germination difficulty due to its seed dormancy. This study was evaluated to find a simple seedling germination method intended for D. stramonium seeds as a traditional healthy detector plant. A factorial experiment organized as completely randomized design (CRD) in triplicate, determining results regarding seedling scarification, gibberellins (GA3) along with potassium nitrate (KNO3) at different concentrations from 0 to 2000 ppm. The particular germination rate along with radicle and plumule length scarified seed had been significantly affected using concentrations of GA3 as well as KNO3. After five days of treatment, radicle and plumule length and the percentage of germination were recorded, uncovering the real key function involving mechanical seed scarification method as being a requirement for germination of D. stramonium. The highest germination rate occurred at a concentration of 100 ppm GA3 and 500 ppm for KNO3 (63%).


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