Effects of Process Parameter on Crude Oil Biodegradation of Palm Oil Mill Effluent using Response Surface Optimization

Document Type : Original Article


1 Department of Chemical Engineering, Faculty of Engineering, Nnamdi Azikiwe University, P.M.B 5025, Awka, Anambra State, Nigeria

2 Department of Science Laboratory Technology, Institute of Management and Technology Enugu P.M.B 1079, Enugu Nigeria


The aim of this study was to investigate the effects of process parameter on crude oil (CO) biodegradation of palm oil mill effluent (POME) by using response surface optimization. Physiochemical characterization of the uncontaminated soil (UCS), crude oil contaminated soils (COCS), and POME were investigated. Further characterization on the POME was done employing the scanning electron microscope (SEM) and Fourier transform infrared (FT-IR). First and second order kinetics models were used to estimate the kinetic parameters. Results obtained indicated that POME contained valuable soil nutrient as it showed a stimulatory effect on the physiochemical properties of the COCS. However, POME was able to degrade 51% of CO with an initial CO concentration of 130 g/L. The first order kinetics proved a better model with a high rate constant, lower biological half-life and R² greater than 0.96. From the optimization process, the quadratic model with 78.8% contribution and R² of 0.993 satisfactorily explained the interactions between the independent variable and the response. The FT-IR spectrum revealed the presence of nitrogen and phosphorous on the surface of POME, while SEM indicated a smooth surface of POME.


1. Rupani P. F., Singh R. P., Ibrahim H., Eas N., 2010. Review of current palm oil mill effluent treatment methods. J World Appl Sci. 11, 70 – 81
2. Madaki S.Y., Seng L., 2013. Palm oil mill effluent (POME) from Malaysia palm oil mill; waste or resource, Inter J Environ Technol. 2, 1138 - 1155
3. Bhatia S., Othman Z., Ahmad A. L., 2007. Coagulation-flocculation process for POME treatment using Moringa oleifera seeds extract: Optimization studies. Chem Eng J. 133, 205 – 212
4. Wu T.Y., Mohammad A.W., Jahim J.M., Anuar N., 2007. Palm oil mill effluent (POME) treatment and bio resources recovery using ultrafiltration membrane: effect of pressure on membrane fouling. Biochem Eng J. 35, 309 – 317
5. Khalid A.R., Mustafa W.A.W., 1992. External benefits of environmental regulation: Resource recovery and the utilization of effluents. The Environmentalist, 12, 277 – 285. doi.org/10.1007/BF01267698.
6. Oviasogie P.O., Aghimien A.E., 2003. Micronutrient status and speciation of copper, iron, zinc and zinc in soil containing palm oil mill effluent. Global J Pure andAppl Sci.9, 71 – 80
7. Wu T.Y., Mohammad A.W., Jahim J.M., Anuar N., 2009. A holistic approach to managing palm oil mill effluent (POME): Biotechnological advances in the sustainable reuse of POME, Biotechnol Adv. 27, 40 – 52. doi: 10.1016/j.biotechadv.2008.08.005
8. Baharuddin A. S., Wakisaka M., Shirai Y., Abd Aziz S., Abdul Rahman N. A., Hassan M. A., 2009. Co-Composting of empty fruit bunches and partially treated palm oil mill effluent in a pilot scale. InterJ Agric Resource. 4, 69 -78
9. Peressutti S.R., Alvaerz H.M., Pucci O.H., 2003. Dynamics of hydrocarbon degrading bacteriocenosi of experimental oil pollution in Patagonian soil, Inter J Bio-deterior Biodegra. 52, 21 – 30.
10. Sayara T., Sarra M., Sanchez A., 2010. Optimization and enhancement of soil Bioremediation by composting using experimental design technique. Biodegrad. 21, 345 – 356
11. American society for testing and materials (ASTM) D4643- 2013. Determination of soil moisture content, US Department of Agriculture.
12. American society for testing and materials (ASTM) D2974- 2014. Determination of soil organic matter contents of soil by Microwave oven heating, US Department of Agriculture.
13. Rutherford P. M., McGill W.B., Arocena J.M., Figueiredo C.T., 2008. Total Nitrogen Soil Sampling and Methods of Analysis, Canadian Society of Soil Science, 3rd Edition, Taylor & Francis Group for CRC Press.
14. American society for testing and materials (ASTM) D4972- 2013. Standard Test Method for pH of Soils, ASTM International, West Conshohocken, www.astm.org doi: 10.1520/D4972.2013
15. American Public Health Association APHA, 2005. Standard method for the determination of water and wastewater, 21st Edition, Total organic carbon (TOC): high temperature combustion method (5319A and 5310B).
16. US Department of Agriculture and the US composting council 2001. Test methods for the determination of available nutrients in compost, Edaphos, International Houston.
17. Riahi K., Safa C., Bechir B.T., 2013. AKinetic modelling study of phosphate adsorptiononto phoenix dactylifera L date palm fibre inbatch mode. J Saudi Chem Soci. In Press.
18. Sayara T., Sarra M., Sanchez A., 2009.Preliminary screening of co substrate for bioremediation of pyrene contaminated soil through composting. J Hazard Mater. 172, 1695-1698
19. Bundy J.G., Paton G.I., Campbell C.D., 2002. Microbial communities in different soiltypes do not converge after diesel contamination. J Appl Microbiol. 92, 276 – 288
20. Alrumman A., Standing D.B., Paton I., 2014. Effect of hydrocarbon contamination on soil microbial community and enzyme activity. J King Saudi Uni. 27, 31- 41
21. Habib M.A.B., Yusoff S.M., Phang K.J., Mohamed S., 1997. Nutritional value ofchironomid larvae grown in palm oil mill effluent and algal culture.  Aquaculture. 158, 95-105
22. Muhrizal S.J., Shamshuddin I., Fauziah M.A., Husni H., 2006. Changes in iron ore pooracid sulphate soil upon submergence Geothermal. 131, 110-122.
23. United State department of Agriculture, Natural Resource Conservation Service (USDA, NRCS) Soil quality kit-guide for educators 2001.
24. Cheraghi M., Sobhanardakani S., Lorstani B., Merrikhpour H., Mosaid H. P., 2015. Biochemical and physical characterization of petroleum hydrocarbon contaminated soil in Tehran. J Chem Health Risks. 5, 199-208
25. Margesin R., Schinner F., 2000. Bioremediation (Natural Attenuation and bio Stimulation) of diesel oil contaminated soil in Apline Glacier skiing area. Appl Environ Microbiol. 67, 3127 - 3133
26. Xu R., Obbard J.P., 2003. Effect of nutrient amendment on indigenous hydrocarbon biodegradation in crude oil contaminated beach sediments. J EnvironQuality. 32, 1234 - 1243.
27. Marinescu M., Toti M., Tanase V., Plopeanu G., 2011. Effect of crude oil pollution on physical and chemical characteristics of soil. Res J Agric. 43,125 – 129
28 Alrawi R.A., Ab Rahman N.N.N., Ahmad A., Ismail N., Mohd Omar A. K., Characterization of oily and Non-Oily Natural Sediments in palm oil mill effluent.  doi.org/10.1155/2013/298958.
29. Grube M., Lin J.G., Lee P.H., Kokorevicha S., 2006. Evaluation of sewage sludge based compost by FT-IR spectroscopy. African J Geo-Chem. 130, 324 - 333
30. Giovanela M., Parlanti E., Sierra S.J., Soldi M.S., Sierra M.D., 2004. Elemental composition FT-IR spectra and thermal behaviour of sedimentary fulvic and humic acid from aquatic and terrestrial environment. J Geo Chem. 38, 255-264
31. Matias M.C., Orden M.U., Sanchez C.G., Urreaga J.M., 2000. Comparative spectroscopy study of modification of cellulose material with different coupling agents. J Appl Poly Sci. 75, 256 – 266
32. Stuart H.B., 2004. Infrared spectroscopy, fundamentals and applications, University of Technology Sydney Australia John Wiley & Sons Ltd.
33. Gailute I., Rackauskiene G., Grigiskis S., 2014. Changes in crude oil hydrocarbon degradation by Arthrobacter Sp. MI and Acinetobacteria Pr82 in selected optimal condition, Biologija. 60, 134 – 141
34. Zappi M.E., Rogers B.A., Teeter C.I., Gunnison D., Bajpai R., 1996. Bio-slurry treatment of soil with low concentration of total petroleum hydrocarbon. J Hazard Mater. 46, 1-12
35. Roling W.F., Milner M.G., Jones D.M., Lee K., Daniel F., Swannell R. J., Head I. M., 2002. Robust hydrocarbon degradation and dynamics of bacterial community during nutrient enhanced oil spill bioremediation. Appl Environ Microbiol. 68, 5537 – 5548
36. Yong-Chao G., Shu-hai G., Jia-ning W., Li D.,Wang H., De Hui-Zang., 2014. Effect of different remediation treatments on crude oil contaminated saline soil.  Chemosphere. 117, 486-493
37. Tanase A.M., Csutak V.T., Pelinesu D., Robertina I., Stoica I., 2012. Phylogenetic analysis of oil polluted soil microbial strain, Romania Biotechnol Lett, 17, 7093 - 7103
38. Hossani A.M., Ngo H.H., Guo W., 2013. Introductory of Microsoft Excel SOLVER Function spread sheet method for isotherm and kinetics modelling of metals biosorption in waste water. J water Sustainability. 3, 223 – 237
39. Chang W., Dyen M., Spagnuolo L., Simon P., Whyte L., Ghoshal S., 2010. Biodegradation of semi and non- semi petroleum hydrocarbon in aged contaminated soil from Sub-Arctic ice. Chemosphere. 80, 319 – 326
40. Kitanovic H.S., Milenovic D., Veeljkovic V.B., 2008. Empirical kinetic models for the resinoid extraction from aerial parts of Saint John’s Wort (Hypericum perforatum L.) Biochem Eng. 41, 1-11
41. Farahat L.A., El-Gendy N.S., 2007. Comparative kinetic study of different bioremediation Process for soil contaminated with petroleum hydrocarbon. J Mater Sci Res India. 4, 269-278.
42. Jorgensen K.S., Puustinen J Suortti, 2000. Bioremediation petroleum hydrocarbon contaminated soil by composting biopile. J Environ Pollut. 107, 245-254
43. Gomez F., 2014. Assessment and Optimization of Ex-Situ Bioremediation of Petroleum contaminated Soil under Cold Temperature Conditions, M.Sc. Thesis, University of Ottawa Canada.
44. Shahavi M.H., Morteza H., Mohsen J., Ghasem N., 2015. Optimization of encapsulated clove oil particle size with biodegradable shell using design expert methodology. Pakistan J Biotechnol. 12,149 - 160
45. Myers R.H., Montgomery D.C., Anderson-Cook C.M., 2009. Response Surface Methodology: Product and Process Optimization Using Designed Experiments. 3rd Edition, John Wiley & Sons, New York.    
46. Debamista N., Rajasimman M., 2013. Optimization and Kinetics study on Biodegradation of atrazine using mixed microorganisms. Alexandria Eng J. 52, 499 – 505
Volume 9, Issue 2
June 2019
Pages 117-132
  • Receive Date: 05 January 2019
  • Revise Date: 13 March 2019
  • Accept Date: 19 April 2019
  • First Publish Date: 01 June 2019