Hematological, Histopathological and Growth Performance Studies on Albino Rats Fed on Mystus gulio Fillet with Formulated Cereal

Document Type: Original Article

Authors

1 Chemistry Discipline, Khulna University, Khulna-9208, Khulna, Bangladesh

2 Chemistry Discipline, Khulna University, Khulna-9208, Khulna, Bangladesh Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Kuantan, Pahang, Malaysia

3 Chemistry Discipline, Khulna University, Khulna-9208, Khulna, Bangladesh Department of Chemistry and Biochemistry, Auburn University, Auburn, Alabama 36849, United States

Abstract

The growth and development of human body is dependent on the protein, carbohydrate, vitamins and minerals. Fishes are one of the major sources of protein, vitamins and minerals for human. This study was conducted to know the effect of Mystus gulio fish fillet on growth performance of albino rats after feeding different concentrations of fish fillet as a protein source with formulated cereal for 32 days. Haematological and histopathological analysis were performed between control group and examined groups of albino rats. It was noticed that the body weight of each experimental albino rat was increasing gradually with increasing fish fillet up to 15% and beyond this the value was decreased. Moreover, the highest protein efficiency ratio (PER) and calorie efficiency ratio (CER) were observed 2.53% and 13.02% respectively for 15% supplied fish fillet. The gained in body weight (GBW) of albino rats in each experimental group depended mainly on supplied fish protein concentration but not on formulated cereal. According to hematological and histopathological studies, no toxicological effect was observed between control group and experimental groups of rats treated with different fish protein concentration for 32 consecutive days. Supplement of 15% fish fillet with formulated cereal was required to produce maximum nutritive values and the Mystus gulio could be considered as protein rich human diet.

Keywords


1. Harper A.E., Benevenga N.J., Wohlhueter R.M., 1970. Effects of ingestion of disproportionate amounts of amino acids. Physiol Rev. 50(3), 428–558.

2. Klahr S., Buerkert J., Purkerson M.L., 1983. Role of dietary factors in the progression of chronic renal disease. Kidney International. 24(5), 579–587.

3. Devaux C., Polzin D.J., Osborne C.A., 1996. What role does dietary protein restriction play in the management of chronic renal failure in dogs? Veterinary Clinics of North America: Small Animal Practice. 26(6), 1247–1267.

4. Barzel U.S., Massey L.K., 1998.  Excess dietary protein can adversely affect bone. J Nutr. 128(6), 1051–1053.

5. Reddy S.T., Wang C.Y., Sakhaee K., Brinkley L., Pak C.Y., 2002. Effect of low-carbohydrate high-protein diets on acid-base balance, stone-forming propensity, and calcium metabolism. Am J Kidney Dis. 40(2), 265–274.

6. Frantz N.Z., Yamka R., Friesen K.G., 2007. The effect of diet and lysine: calorie ratio on body composition and kidney health in geriatric cats. J App Res in Veterinary Medicine. 5(1), 25–36.

7. Farida K.A., 1982. Textbook of Pathology, Lubdhok Prakashani:  Dhaka, Bangladesh.

8. Chukwu L.O., 2006. Histophysiological and basal metabolic responses of albino rat, rattus norvegicus (L.) exposed to aqueous pepper extracts. Afri J Biochem. 5(13), 1279–1283.

9. Wikipedia. Histopathology. https:// en.wikipedia. org/wiki/Histopathology (Accessed October 11, 2018).

10. Ara H., Jesmin M., Rahman S.M., Karim K.M.R., Rahaman M.S., 2006. Proximate and mineral composition of four tengra (Mystus S.P.) fishes of Bangladesh. KU. Studies. 7(2), 51-54.

11. Safi M., 2003. Bangladesh Fisheries, Academic Press and Publishers Limited: Dhaka, Bangladesh.

12. De H.N., Islam A., Mollah Y., 1965. Utilization of protein of fish flour or fish protein concentrate under different dietary levels in body protein synthesis and fat deposition in relation to growth of immature and adult rats. Scientific Res. 2(3), 118–126.

13. Kazi K.A., 1982. Practical Pathology, Kazi Talib Al- Mamun (Ashi): Dhaka, Bangladesh.

14. Alagbaoso S.O., Nwosu J.N., Njoku N.E., Okoye E.C., Eluchie C.N., Agunwa I.M., 2017. Haematology and growth study of albino rats fed varying inclusions of cooked canavalia plagiosperma piper seed meal based-diets. J Food & Nutri Res. 5(9), 649–658.

15. Rao P., Sujatha D., Raj K.R., Vishwanatha S., Narasimhamurthy K., Saibaba P., Rao D.N., Divakar S., 2000. Safety aspects of residual ß–cyclodextrin in egg treated for cholesterol removal. J Eur Food Res Technol.211(6), 393–395.

16. Jayaraman J., 1981. Laboratory Manual in Biochemistry, Wiley Eastern Ltd: New Delhi, India.

17. Mitruka B.M., Ramsley, H.M., 1981. Clinical Biochemical and Hematological Reference Values in Normal Experimental Animals and Normal Humans, 2nd ed., Year Book Medical Publishers Inc: 35 East Wacker drive, Chicago.