Biostimulation to improve the dye biodegradation of organic dyes by activated sludge



In this work, biodegradation of organic pollutants by activated sludge (AS) in the presence of glucose (2 g/L) as an additional carbon source was studied. The AS (without pre-acclimation) was freely suspended under aerobic conditions. Three organic dyes representative of the Algerian textile industry were selected: Cibanon Navy (CN), Solophenyl Scarlet (SS) and Cibacron Green (CG). The results showed that after 10 days of incubation, AS displayed good biodegradation capabilities achieving removal percentages ranging from 50.3% to 89.4% and reduction in COD ranging from 93.1% to 98.3%. Particularly, the textile dye CN was removed up to 89% with high reduction in COD (94.7%). The microbial development stimulated by glucose achieved therefore efficiently the discoloration of contaminated solutions and pollutant degradation. Although it is assumed that dyes can be degraded only under anaerobic conditions, the wastewater treatment using AS appears therefore suitable to the removal of different types of textile dyes before final discharge.

  1. Shah M., 2014. Effective treatment systems for azo dye degradation: A joint venture between physico-chemical & microbiological process. In-ter. J. Environ. Bioremed. Biodegrad. 2(5), 231-242.
  2. Tan L., Ning S., Xia H., Sun J., 2013. Aerobic decolorization and mineralization of azo dyes by a microbial community in the absence of an external carbon source. Intern. Biodeter. Biodegrad. 85, 210-216.
  3. Hasanzadeh M., Hadavi Moghadam B., 2013. Electrospun nanofibrous membranes as potential adsorbents for textile dye removal-A review. J. Chem. Health Risks 3(2), 15-26.
  4. Khalid A., Arshad M., Crowley D., 2010. Biodegra-dation of Azo Dyes. The Handbook of Environ. Chem. 9, 1-37.
  5. Li Song, Yifan Shao, Shuxiang Ning, Liang Tan, 2017. Performance of a newly isolated salt-tolerant yeast strain Pichia occidentalis G1 for degrading and detoxifying azo dye. Bioresour. Technol. 233, 21ââ‚‌“29
  6. Ortiz-Monsalve S., Dornelles J., Poll E., Ramirez-Castrillón M., Valente P., Gutterres M., 2017. Biodecolourisation and biodegradation of leather dyes by a native isolate of Trametes villosa. Pro-cess Safety Environ. Protec. 109, 437-451.
  7. Sahinkaya E., Dilek F.B., 2007. Biodegradation kinetics of 2, 4-dichlorophenol by acclimated mixed cultures. J. Biotechnol. 127, 716-726.
  8. Salehi Z., Yoshikawa H., Mineta R., Kawase Y., 2011. Aerobic biodegradation of p-nitrophenol by acclimated waste activated sludge in a slurry bubble column. Process Biochem. 46, 284-289.
  9. Porwal H.J., Mane A.V., Velhal S.G., 2015. Biodegradation of dairy effluent by using microbial isolates obtained from activated sludge. Water Resourc. Indust. 9, 1-15.
  10. Brosillon S., Djelal H., Merienne N., Amrane A., 2008. Innovative integrated process for the treatment of azo dyes: coupling of photocatalysis and biological treatment. Desalination 222, 331-339.
  11. Chen B., Wang X., Wang C., Jiang W., Li S., 2011. Degradation of azo dye direct sky blue 5B by sonication combined with zero-valent iron. Ultrason. Sonochem. 18, 1091-1096.
  12. Chebli D., Fourcade F., Brosillon S., Nacef S., Amrane A., 2011. Integration of photocatalysis and biological treatment for azo dye removal-application to AR183. Environ. Technol. 32(5), 507-514.
  13. Gomes A.C., Fernandes L.R., Simões R.M.S., 2012. Oxidation rates of two textile dyes by ozone: Effect of pH and competitive kinetics. Chem. Eng. J. 189-190, 175-181.
  14. Antonin V.S., Garcia-Segura S., Santos M.C., Enric Brillas E., 2015. Degradation of Evans Blue diazo dye by electrochemical processes based on Fentonââ‚‌™s reaction chemistry. J. Electroanal. Chem. 747, 1-11.
  15. Zeghioud H., Khellaf N., Amrane A., Djelal H., W. Elfalleh, Assadi A. A., Rtimi S., Photocatalytic performance of TiO2 impregnated polyester for the degradation of Reactive Green 12: implica-tions of the surface pretreatment and the micro-structure. J. Photochem. Photobiol. A: Chemis-try, 346, 493-501.
  16. Sen S.K., Raut S., Bandyopadhyay P., Raut S., 2016. Fungal decolouration and degradation of azo dyes: A review. Fungal Biology Reviews 30, 112-133.
  17. Mesquita D.P., Amaral A.L., Ferreira E.C., 2013. Activated sludge characterization through mi-croscopy: A review on quantitative image analy-sis and chemometric techniques. Anal. Chim. Acta 802, 14-28.
  18. Wilén B-M., Lumley D., Mattsson A., Mino T., 2008. Relationship between floc composition and flocculation and settling properties studied at a full scale activated sludge plant. Water Res. 42(16), 4404-4418.
  19. Lee S-H., Kang H-J., Park H-D., 2015. Influence of influent wastewater communities on temporal variation of activated sludge communities. Water Res. 73, 132-144.
  20. Christie R.M., 2007. Environmental aspects of textile dyeing. Woodhead Publishing Limited, Cambridge, England, 233 pages.
  21. Gokcen F., Ozbelge T.A., 2006. Pre-ozonation of aqueous azo dye (Acid Red-151) followed by activated sludge process. Chem. Eng. J. 123, 109-115.
  22. Jafari N., Reza Saoudi M., Kasra-Kermanshahi R., 2014. Biodecolorization of textile azo dyes by isolated yeast from activated sludge: Issatchenkia orientalis JKS. Annals Microbio. 64, 475-482.
  23. Bajaj M., Gallert C., Winter J., 2008. Biodegrada-tion of high phenol containing synthetic wastewater by an aerobic fixed bed reactor. Bioresour. Technol. 99, 8376-8381.
  24. Semrany S., Favier L., Djelal H., Taha S., Amrane A., 2012. Bioaugmentation: Possible solution in the treatment of Bio-Refractory Organic Com-pounds (Bio-ROCs). Biochem. Eng. J. 69, 75-86.
  25. Duchene Ph., Cotteux E., 1993. Les éléments les plus significatifs de la microfaune des boues ac-tivées. TSM 9, 771-477.
  26. Yang Q., Tao L., Yang M., Zhang H., 2008. Effects of glucose on the decolorization of Reactive Black 5 by yeast isolates. J. Environ. Sci. 20(1), 105-108.
  27. Bromley-Challenor K.C.A., Knapp J.S., Zhang Z., Gray N.C.C., Hetheridge M.J., Evans M.R., 2000. Decolorization of an azo dye by unacclimated activated sludge under anaerobic conditions. Water Res. 34(18), 4410-4418.
  28. Kapdan I.K., Kargi F., 2002. Simultaneous biodeg-radation and adsorption of textile dyestuff in an activated sludge unit. Process Biochem. 37(9), 973-981.
  29. Kumar K., Dastidar M.G., Sreekrishnan T.R., 2009. Effect of process parameter on aerobic decolorization of reactive azo dye using mixed culture. World Acad. Sci. Eng. Technol. 58, 952-955.
  30. Li H., Tan L., Ning S., He M., 2015. Reactor per-formance and microbial community dynamics during aerobic degradation and detoxification of Acid Red B with activated sludge bioaugmented by a yeast Candida tropicalis TL-F1 in MBR. Inter. Biodeter. Biodegrad. 104, 149-156.
  31. Padmavathy S., Sandhya S., Swaminathan K., Subrahmanyam Y.V., Chakrabarti T., Kaul S.N., 2003. Aerobic Decolorization of Reactive Azo Dyes in Presence of Various Cosubstrates. Chem. Biochem. Eng. Q. 17(2) 147-151.
  32. Punzi M., Anbalagan A., Borner R.A., Svensson B.M., Jonstrup M., Mattiasson B., 2015. Degra-dation of a textile azo dye using biological treatment followed by photo-Fenton oxidation: Evaluation of toxicity and microbial community structure. Chem. Ing. J. 270, 290-299.
  33. Franca R.D.G., Vieira A., Mata A.M.T., Carvalho G.S., Pinheiro H.M., Lourenço N.D., 2015. Ef-fect of an azo dye on the performance of an aer-obic granular sludge sequencing batch reactor treating a simulated textile wastewater. Water Res., in press.
  34. Ben Mansour H., Corroler D., Barillier D., Ghedira K., Chekir L., Mosrati R., 2007. Evaluation of genotoxicity and pro-oxidant effect of the azo dyes: Acids yellow 17, violet 7 and orange 52, and of their degradation products by Pseudomo-nas putida mt-2. Food Chem. Toxicol. 45, 1670-1677.
  35. Ben Mansour H., Mosrati R., Corroler D., Ghedira K., Barillier D., Chekir L., 2009. In vitro muta-genicity of Acid Violet 7 and its degradation products by Pseudomonas putida mt-2: Correla-tion with chemical structures. Environ. Toxicol. Pharmacol. 27, 231-236.