Ovarian Expression of Sox2 during Mouse Estrous Cycle

Authors

1 School of Biology, Damghan University, Damghan, Iran

2 School of Biology, Damghan University, Damghan, Iran Institute of Biological Sciences, Damghan University, Damghan, Iran

Abstract

The transcriptional factor Sox2 regulates the expression of some of the developmental genes, which are essential for the maintenance of pluripotency of stem cells. Sox2 also expresses in the female gamete during folliculogenesis, but its role remains ambiguous. The aim of this study was to investigate the expression of Sox2 in the mice ovarian tissue during different stage of estrous cycle. Adult National Medical Research Institute (NMRI) mice were considered as pro-estrous, estrous, met-estrous and di-estrous based on the cell type of the vaginal smear. Immunohistochemical staining of Sox2 marker was performed in mice ovarian tissue. Immunohistochemical staining revealed the expression of Sox2 in the cytoplasm of corpus luteum cells, stromal cells and oocyte. Our results suggest that adult mice ovaries accommodate cells carrying stem cell features.

Keywords

References

  1. Bareiss P.M., Paczulla A., Wang H., Schairer R., Wiehr S., Kohlhofer U., Rothfuss O.C., Fischer A., Perner S., Staebler A., Wallwiener D., Fend F., Fehm T., Pichler B., Kanz L., Quintanilla-Martinez L., Schulze-Osthoff K., Essmann F., Lengerke C., 2013. Sox2 expression associates with stem cell state in human ovarian carcinoma. Cancer Res. 73, 5544-5555.
  2. Pesce M., Schöler H.R., 2000. Control of totipotency and germline determination. Molecular Reproduction and Development. 55, 452-457.
  3. Avilion A.A., Nicolis S.K., Pevny L.H., Perez L., Vivian N., Lovell-Badge R., 2003. Multipotent cell lineages in early mouse development depend on Sox2 function. Genes Dev. 17, 126-140.
  4. Bagheripoor N., Zavareh S., Ghorbanian M.T., Seyed Hassan P., Seyed Reza M., 2017. In Veterinary Research Forum (Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. In Press.
  5. Choobineh K., Zavareh S., Salehnia M., Ghorbanian M.T., 2016. In Veterinary Research Forum (Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. pp. 181.
  6. Singletary S.J., Kirsch A.J., Watson J., Karim B.O., Huso D.L., Hurn P.D., Murphy S.J., 2005. Lack of correlation of vaginal impedance measurements with hormone levels in the rat. Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science. 44, 37-42.
  7. Otsubo T., Akiyama Y., Yanagihara K., Yuasa Y., 2008. Sox2 is frequently downregulated in gastric cancers and inhibits cell growth through cell-cycle arrest and apoptosis. Br J Cancer. 98, 824-831.
  8. Go M.J., Takenaka C., Ohgushi H., 2008. Forced expression of Sox2 or Nanog in human bone marrow derived mesenchymal stem cells maintains their expansion and differentiation capabilities. Exp Cell Res. 314, 1147-1154.
  9. Samardzija C., Quinn M., Findlay J.K., Ahmed N., 2012. Attributes of Oct4 in stem cell biology: perspectives on cancer stem cells of the ovary. J Ovarian Res. 5, 37-49
  10. Virant-Klun I., Stimpfel M., Skutella T., 2012. Stem cells in adult human ovaries: from female fertility to ovarian cancer. Curr Pharm Des. 18, 283-292.
  11. Boyer L.A., Lee T.I., Cole M.F., Johnstone S.E., Levine S.S., Zucker J.P., Guenther M.G., Kumar R.M., Murray H.L., Jenner R.G., 2005. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. 122, 947-956.
  12. Chew J.L., Loh Y.H., Zhang W., Chen X., Tam W.L., Yeap L.S., Li P., Ang Y.S., Lim B., Robson P., 2005. Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells. Molecular and Cellular Biology, 25, 6031-6046.
  13. Wang X., Dai J., 2010. Concise review: isoforms of OCT4 contribute to the confusing diversity in stem cell biology. Stem Cells, 28, 885-893.
Journal of Chemical Health Risks

Volume 7, Issue 2
Spring 2017

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History

  • Receive Date: 26 April 2017
  • Revise Date: 02 December 2020
  • Accept Date: 29 October 2018

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