Biochemical Responses of Two Soybean (Glycine max) Varieties to Aluminum Stress in Nutrient Solution


Department of Botany, Faculty of Biology, Damghan University, Damghan, Iran


Aluminum toxicity is the most widespread form of metal toxicity to plants in soil acids, initially causing inhibition of root elongation and blocks absorption of water and nutrients. According to this fact that soybean has been widely used in industry, this study investigated the effects of aluminum toxicity on biochemical factors in two varieties of Williams and Katoul of soybean plant. The study was carried out in a randomized design with aluminium (0, 200, 500, 700 µM) treatments and four replications in hydroponic culture. Results of biochemical tests showed that aluminum reduced the content of photosynthetic pigments, flavonoids, phenolic compounds, anthocyanins and reduced sugars in both cultivars of soybean. The proline content decreased with increasing aluminum in var. williams, but at var. katoul increased. It seems that G. max var. katoul suffers less than var. Williams. As regards, proline accumulation under Al stress to be generally higher in G. max var. katoul; hence, these results suggest that var. katoul is more resistant than var. Williams.


  1. Ghrefat H., Yusuf N., 2006. Assessing Mn, Fe, Cu, Zn, and Cd pollution in bottom sediments of Wadi Al-Arab Dam, Jordan. Chemosphere. 65(11), 2114-2121.
  2. Yalcin M.G., Battaloglu R., Ilhan S., 2007. Heavy metal sources in Sultan Marsh and its neighborhood, Kayseri, Turkey. Environ Geol. 53(2), 399-415.
  3. May H.M., Nordstrom D.K. Assessing the solubilities and reaction kinetics of aluminous minerals in soils. In: Soil acidity, Eds., Springer Berlin Heidelberg, 1991. pp. 125-148
  4. Kochian L.V., Pineros M.A., Hoekenga O.A. The physiology, genetics and molecular biology of plant aluminum resistance and toxicity. In: Root Physiology: From Gene to Function, Eds., Springer Netherlands. 2005. pp. 175-195
  5. Panda S.K., Matsumoto H., 2007. Molecular physiology of aluminum toxicity and tolerance in plants. Bot Rev. 73(4), 326-347.
  6. Kinraide T.B. Identity of the rhizotoxic aluminium species. In: Plant-soil interactions at low pH, Eds., Springer Netherlands, 1991. pp. 717-728.
  7. Delhaize E., Ma J.F., Ryan P.R., 2012. Transcriptional regulation of aluminium tolerance genes. Trends Plant Sci. 17(6), 341-348.
  8. De Macêdo C.E.C., Jan V.V.S., Kinet J.M., Lutts S., 2009. Effects of aluminium on root growth and apical root cells in rice (Oryza sativa L.) cultivars. Reliability of screening tests to detect Al resistance at the seedling stage. Acta Physiol Plant. 31(6), 1255-1262.
  9. Sharma P., Dubey R.S., 2007. Involvement of oxidative stress and role of antioxidative defense system in growing rice seedlings exposed to toxic concentrations of aluminum. Plant Cell Rep. 26(11), 2027-2038.
  10. Pandey P., Srivastava R.K., Dubey R.S., 2013. Salicylic acid alleviates aluminum toxicity in rice seedlings better than magnesium and calcium by reducing aluminum uptake, suppressing oxidative damage and increasing antioxidative defense. Ecotoxicol. 22(4), 656-670.
  11. Yamamoto Y., Kobayashi Y., Matsumoto H., 2001. Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots. Plant Physiol. 125(1), 199-208.
  12. Clark R.B., Pier P.A., Knudsen D., Maranville J.W., 1981. Effect of trace element deficiencies and excesses on mineral nutrients in sorghum. J Plant Nutr. 3(1-4), 357-374.
  13. Foy C.D., Fleming A.L., 1982. Aluminium tolerances of two wheat cultivars related to nitrate reductase activities. J Plant Nutr. 5, 1313ââ‚‌“1333.
  14. Taylor G.J., 1991. Current views of the aluminum stress response: The physiological basis of tolerance. Curr Topics Plant Biochem Physiol. 10, 57ââ‚‌“93.
  15. Lichtenthaler H.K., 1987. Chlorophyll and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol. 148, 350-382
  16. Krizek D.T., Britz S.J., Mirecki, R.M., 1998. Inhibitory effects of ambient levels of solar UVââ‚‌A and UVââ‚‌B radiation on growth of cv. New Red Fire lettuce. Physiol Plant. 103(1), 1-7.
  17. Wagner G.J., 1979. Content and
  18. vacuole / extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiol. 64(1), 88-93.
  19. Seevers P.M., Daly J.M., 1970. Studies on wheat stem rust resistance control at sr6 locus. 1-the role of phenolic compounds. Phytopathology. 6, 1322-1328.
  20. Somogyi M., 1952. Notes on sugar determination. J Biol Chem. 195(1), 19-23.
  21. Bates L.S., Waldren R.P., Teare I.D., 1973. Rapid determination of free proline for water-stress studies. Plant Soil. 39(1), 205-207.
  22. Ziaei N., Rezaiatmand Z., Ranjbar M., 2014. Study of Aluminum Toxicity on Photosynthetic Pigment, Soluble Sugars and Proline Contents in Two Sunflower Varieties. Res Crop Ecophysiol. 9/1(2), 105-113. (In Persian).
  23. Malekzadeh P., Mehr R.S., Hatamnia A.A., 2015. Effects of aluminum toxicity on maize (zea mays L.) seedlings. Iran J Plant Physiol. 5(2), 1289-1296.
  24. Haag-Kerwer A., Schäfer H.J., Heiss S., Walte C., Rausch T., 1999. Cadmium exposure in Brassica juncea causes a decline in transpiration rate and leaf expansion without effect on photosynthesis. J Exp Bot. 50(341), 1827-1835.
  25. Rout G., Samantaray S., Das P., 2001. Aluminium toxicity in plants: a review. Agronomie. 21(1), 3-21.
  26. Macfarlane G.R., Burchett M.D., 2001. Photosynthetic pigments and peroxidase activity as indicators of heavy metal stress in the Grey mangrove, Avicennia marina (Forsk.) Vierh. Mar Pollut Bull. 42(3), 233-240.
  27. Ngo T., Zhao Y., 2005. Retaining Green Pigments on Thermally Processed Peelsââ‚‌on Green Pears. J Food Sci. 70(9), 568-574.
  28. Lau T.S.L., Eno E., Goldstein G., Smith C., Christopher D.A., 2006. Ambient levels of UV-B in Hawaii combined with nutrient deficiency decrease photosynthesis in near-isogenic maize lines varying in leaf flavonoids: Flavonoids decrease photoinhibition in plants exposed to UV-B. Photosynthetica. 44(3), 394-403.
  29. Fargašová A., 1998. Root growth inhibition, photosynthetic pigments production, and metal accumulation in Sinapisalba as the parameters for trace metals effect determination. Bull Environ Contam Toxicol. 61(6), 762-769.
  30. Singh P.K., Tewari R.K., 2003. Cadmium toxicity induced changes in plant water relations and oxidative metabolism of Brassica juncea L. plants. J Environ Biol. 24(1), 107-112.
  31. Sai Kachout S., Ben Mansoura A., Ennajah A., Leclerc J.C., Ouerghi Z., Karray Bouraoui N., 2015. Effects of metal toxicity on growth and pigment con-tents of annual halophyte (A. hortensis and A. rosea). Int J Environ Res. 9(2), 613-620.
  32. Tohidi Z., Baghizadeh A., Enteshari S., 2015. The Effects of Aluminum and Phosphorous on some of Physiological Characteristics of Brassica napus. J Stress Physiol Biochem. 11(1), 16-28
  33. Jenkins G.I., 1999a. Regulation of phenylpropanoid and flavonoid biosynthesis genes by UV-B in Arabidopsis. In: Smallwood MF, Calvert CM, Bowles DJ, Eds., Plant responses to environmental stress. Oxford, UK: Bios Scientific Publishers Ltd, pp. 9ââ‚‌“15
  34. Najafi F., Khavari nejad R., Mohamadi F., 2013 .Effect of Indole acetic acid impact on the amount of protein, carbohydrates and gas exchange in soybean under aluminum chloride stress. J Sci Kharazmi Uni. 12(3), 581-600. (In Persian).
  35. Peixoto H.P., Da Matta F.M., Da Matt J.C., 2002. Responses of the photosynthetic apparatus to aluminum stress in two sorghum cultivars. J Plant Nutr. 25(4), 821-832.
  36. Chen L-S., Qi Y.P., Smith B.R., Liu X.H., 2005. Aluminum- induced decrease in CO2 assimilation in citrus seedling is unaccompanied by decreased activities of key enzymes involved in CO2 assimilation. Tree Physiol. 25, 317-324.
  37. Elhabiri M., Figueiredo P., Toki K., Brouillard R., 1997. Anthocyaninââ‚‌“aluminium andââ‚‌“gallium complexes in aqueous solution. J Chem Soc Perkin Trans. 2(2), 355-362.
  38. Hale K.L., McGrath S.P., Lombi E., Stack S.M., Terry N., Pickering I.J., George G.N., lon-Smits E.A., 2001. Molybdenum Sequestration in Brassica Species. A Role for Anthocyanins? Plant Physiol. 126(4), 1391-1402.
  39. Neill S.O., Gould K.S., Kilmartin P.A., Mitchell K.A., Markham K.R., 2002. Antioxidant capacities of green and cyanic leaves in the sun species, Quintinia serrata Funct Plant Biol. 29(12), 1437-1443.
  40. Mirzayan F., Enteshari S.h., Hajbagheri S., 2012. Role of Mycorrhizal Fungi on antioxidant compounds of basil (Ocimum basilicumin L.) in contaminated lands with aluminum. The first national conference on plant stress, In: University of Esfahan, Iran. (In Persian).
  41. Gitz D.C., Liu-Gitz L., Mcclure J.W., Huerta A., 2004. Effects of a PAL. Inhibitor on Phenolicaccumulation and UV-B tolerance in Spiodela intermedia (Koch). J Exp Bot. 55, 919-927.
  42. Da Cruz F.J.R., da Silva Lobato A.K., da Costa R.C.L., dos Santos Lopes M.J., Neves H.K.B., de Oliveira Neto C.F., da Silva M.H.L., dos Santos Filho B.G., de Lima Junior J.A., Okumura R.S., 2011. Aluminum negative impact on nitrate reductase activity, nitrogen compounds and morphological parameters in sorghum plants. Aust J Crop Sci. 5(6), 641.
  43. Demir Y., 2000. Growth and Proline Content ongerminating wheat genotypes under ultravioletlight. Turk J Bot. 24, 67-70.
  44. Venekamp, J.H., 1989. Regulation of cytosol acidity in plants under conditions of drought. Physiol Plant. 76(1), 112-117.
  45. Sawhney V., Sheoran I.S., Singh R., 1990. Nitrogen fixation, photosynthesis and enzymes of ammonia assimilation and ureide biogenesis in nodules of mung-bean (Vigna radiata) grown in presence of cadmium. Indian J Exp Biol. 28(9), 883-886.
  46. Saradhi P.P., Mohanty P., 1993. Proline in relation to free radical production in seedlings of Brassica juncea raised under sodium chloride stress. Plant Soil. 155(1), 497-500.
  47. Ashraf M., Foolad M., 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot. 59(2), 206-216.
  48. Kasai Y., Kato M., Aoyama J., Hyodo H., 1998. Ethylene production and increase in 1-amino-cyclopropane-1-carboxylate oxidase activity during senescence of broccoli florets. In: ISHS Acta Horticulturae 464: International Postharvest Science Conference Postharvest 96. Eds., Bieleski R., Laing W., Clark C., Taupo New Zealand, 1998. pp. 153-158.
  • Receive Date: 17 September 2016
  • Revise Date: 23 May 2022
  • Accept Date: 29 October 2018
  • First Publish Date: 29 October 2018