Evaluation of Antimicrobial Effect of Cinnamomum verum Methanolic Extract and Essential Oil: A Study on Bio-preservative in Ketchup Sauce

Authors

1 Department of Food Science and Technology, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Food Science and Technology, Faculty of basic sciences, Islamic Azad University of Varamin- pishva, Tehran, Iran

3 Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran

Abstract

Use of natural antimicrobial compounds extracted from plants such as cinnamon as preservative to extend the shelf life has gain much attention. In this study, we evaluated the antimicrobial activity and bio- preservative potential of the methanolic extract of Cinnamomum verum bark and its oil against Bacillus subtilis, Pseudomonas aeruginosae and Escherichia coli in ketchup sauce. In order to evaluate the Minimum Inhibitory Concentration (MIC) of essential oil and methanolic extract from the bark of C. verum, agar dilution method was performed. Then the effective inhibitory concentrations were evaluated on growth of test bacteria in ketchup sauce at 4 °C and room temperature in different storage times (1, 7, 14 and 30 days). In addition, the sensory quality of treated ketchups was assessed. This study showed bacteriostatic effect of the essential oils and methanolic extract on all tested bacteria. The best treatment in ketchup sauce was obtained on days 14 at 4 °C in concentrations of 1500 µg/ml essential oil. In sensory evaluation, the sample containing 1000 µg/ml essential oil had higher score in odor, taste and overall acceptability than other treated samples (P<0.05). This study shows that cinnamon oil is a more potent antimicrobial agent than cinnamon extract and it has the potential to be used as food preservative and is recommended for quality attributes enhancement.

Keywords

References

Bondi M., Messi P., Halami M., 2014. Emerging Microbial Concerns in Food Safety and New Control Measures. BioMed Res Int. 1-3. Article ID 251512.
Anand S.P., Sati N., 2013. Artificial preservatives and their harmful effects: looking toward nature for safer alternatives. Inter J Pharma Sci Res. 4, 2496-2501.
Negi P.S., 2013. Plant extracts for the control of bacterial growth: Efficacy, stability and safety issues for food application. Int J Food Microbiol1. 156 (1), 7ââ‚‌“17.
Gislene G.F., Locatelli J., Freitas P., 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Braz J Microbiol. 31(4), 247-256.
Celikel N., Kavas G., 2008. Antimicrobial Properties of Some Essential Oils against Some Pathogenic Microorganisms. Czech J Food Sci. 26(3), 174ââ‚‌“181.
Ranasinghe P., Pigera S., Premakumara G.A., 2013. Medicinal properties of ââ‚‌˜trueââ‚‌™ cinnamon (Cinnamomum zeylanicum): a systematic review. BMC Complement Altern Med. 13, 275-284.
Al-Mariri A., Saour G., Hamou R., 2012. In Vitro Antibacterial Effects of Five Volatile Oil Extracts Against Intramacrophage Brucella Abortus 544. Iran J Med Sci. 37, 119ââ‚‌“125.
Mith H., Duré R., Delcenserie V., 2014. Antimicrobial activities of commercial essential oils and their components against food-borne pathogens and food spoilage bacteria. Food Sci Nutr. 2(4), 403ââ‚‌“416.
Rasheeha N., Iftikhar H., Tawab A., 2013. Antimicrobial activity of the bioactive components of essential oils from Pakistani spices against Salmonella and other multi-drug resistant bacteria. BMC Complement Altern Med. 13, 265-274.
- Sienkiewicz M., GÅ‚owacka A., Kowalczyk E., 2014. The Biological Activities of Cinnamon, Geranium and Lavender Essential Oils. Molecules. 19(2), 20929-20940.
Muthuswamy S., Rupasinghe H.P.V., Stratton G.W., 2008. Antimicrobial effect of cinnamon bark extract on Escherichia coli O157:H7, Listeria innocua and fresh-cut apple slices. J Food Safety. 28(4), 534- 549.
Yuste J., Fung D.Y., 2002. Inactivation of Listeria monocytogenes Scott A 49594 in apple juice supplemented with cinnamon. J Food Prot. 65(10), 1663-1666.
Mazimba O., Wale K., Tebogo E., Tebogo E., Kwape Shetonde O., 2015. Cinnamomum verum: Ethylacetate and methanol extracts antioxidant and antimicrobial activity. J Med Plants Studies. 3(3), 28-32.
Su L., Yin J., Charles D., Zhou K., Moore J., 2007. Total phenolic contents chelating capacities, and radical scarenging properties of black peppercorn, nutmeg, rosehip, cinnamon and cregano leaf. J Food Chem. 100(3), 990-997.
Parekh J., Jadega D., Chanda S., 2005. Efficacy of Aqueous and Methanol Extracts of Some Medicinal Plants for Potential Antibacterial Activity. Turk J Biol. 29, 203-210.
Subramanian P.A., Gebrekidan A., Nigussie K., 2012. Yield, Contents and Chemical Composition Variations in the Essential oils of Different Eucalyptus globulus trees from Tigray, Northern Ethiopia. J Pharm Biomed Sci. 17(17), 1-6.
Andrews J.M., 2001. Determination of minimum inhibitory concentrations. J Antimicro Chemo. 48 (1), 5-16.
Reddy V., Urooj A., Kumar A., 2005. Evaluation of antioxidant activity of some plant extracts and their application in biscuits. J Food Chem. 90 (1-2), 317 - 21.
Gende L.B., Floris I., Fritz R., Javier M., 2008. Antimicrobial activity of cinnamon (Cinnamomum zeylanicum) essential oil and its main components against paenibacillus larvae from argentine,ââ‚‌ Bull Insectol. 61(1), 1ââ‚‌“4.
Prabuseenivasan S., Jayakumar M., Ignacimuthu S., 2006. In vitro antibacterial activity of some plant essential oils. BioMed Central Complementary Alternative Med. 6, 39-47.
Mang B., Wolters M., Schmitt B., 2006. Effects of a cinnamon extract on plasma glucose, HbA1c, and serum lipids in diabetes mellitus type 2. Euro J Clin Investig. 36(5), 340ââ‚‌“344.
Irshad M., Zafaryab M., Singh M., 2012. Comparative analysis of the antioxidant activity of Cassia fistula extracts. Int J Med Chem. 10, 1155-1161.
Rota C., Carramiñana J., Burillo J., Herrera A., 2004. In vitro antimicrobial activity of essential oils from aromatic plants against selected foodborne pathogens. J Food Protection. 67(6), 1252ââ‚‌“1256.
Harsha N., Sridevi V., Lakshmi M.V.V.C., Lakshmi C., Sataya D., 2013. Phytochemical analysis of some selected spices. Int J Innov Res Sci Eng Technol. 2(11), 6618-6621.
Rakshit M., Ramalingam C., 2011. In-vitro antibacterial and antiodixant activity of Cinnamomum verum (Cinnamon) aqueous bark extract in reference to its total phenol content as natural preservative to food. Int J Biotechnol. 8(4), 529-537.
Mazimba O., Kabo W., Tebogo E., Shetonde O., Bkolo M., 2015. Cinnamomum verum: Ethylacetate and methanol extracts antioxidant and antimicrobial activity. J Med Plants Studies. 3(3), 28-32.
Matan N., Rimkeeree H., Mawson A J., Chomprida P., Haruthaithanasan V., Parker M., 2006. Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. Int J Food Microbiol. 107(2), 180ââ‚‌“185.
Tayel A.A., El-Tras W.F., Moussa S.H., El-Sabbagh S.M., 2012. Surface Decontamination and Quality Enhancement in Meat Steaks Using Plant Extracts as Natural Biopreservatives. Foodborne Pathog Dis. 9(8), 755-61.
Holley R.A., Patel D., 2005. Improvement in shelf life and safety of perishable foods by plant essential oils and smoke antimicrobials. J Food Microbiol. 22(4), 273-92.

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History

  • Receive Date: 06 June 2016
  • Revise Date: 18 April 2021
  • Accept Date: 29 October 2018

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