Development of Directly Suspended Droplet Micro Extraction Method for Extraction of Organochlorine Pesticides in Water Samples

Author

Department of Chemistry, Young Researchers and Elite Club, Ilam Branch, Islamic Azad University, Ilam, Iran

Abstract

A simple and efficient directly suspended droplet micro extraction in conjunction with gas chromatography-electron capture detector (GC-ECD) has been developed for extraction and determination of organochlorine pesticides (OCPs) from water samples. In this technique a micro drop of 1-dodecanol is delivered to the surface of an aqueous sample while being agitated by a stirring bar in the bulk of solution. Factors relevant to the extraction efficiency were studied and optimized. The optimized extraction conditions were extraction solvent: 1-dodecanol; extraction temperature: 60◦C; NaCl concentration: 0.5M; solvent extraction volume: 10 µL; stirring rate: 800rpm and the extraction time: 20 min. The detection limits of the method were in the range of 0.066–1.85 ngL−1, relation standard deviation (n=5) range were 0.102 - 0.964. A good linearity (r 2 ≥0.995) and a relatively broad dynamic linear range (25–2600ng.L−1) were obtained and recoveries of method were in the range of 90.729% - 102.343%. Finally, the proposedmethod was successfully utilized for pre concentration and determination of OCPs in different real samples.We successfully developed a method based on the DSDME technique combined with capillary GC-ECD for the analysis of OCPs in the water samples and compared with the conventional sample preparation method such as LPME.   

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