Characterization of Chemical Composition and Antioxidant Properties of Trachyspermum ammi Seed as a Potential Medicinal Plant

Authors

1 Department of Basic Sciences, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran

2 Department of Chemistry, Hakim Sabzevari University, Sabzevar, Iran

3 Traditional Medicine Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran

Abstract

In this study, the essential oil obtained by hydrodistillation of the seed of Trachyspermum ammi (Umbelliferae), growing wild in Sabzevar, Khorasan Razavi province (Iran), were analyzed by GC and GC/MS. The yield of total volatiles was 2.3% (w/w). Forty four compounds representing 91.6% of the aerial parts oil were identified.  The main components of the oil were Hexadecanoic acid (27.5%), ethyl linoleate (8.5%), 6-methyl-α-ionone (8.0%), isobutyl phthalate (5.8%), α-cadinol (4.7%), germacrene D (4.3%) and δ-cadinene (3.5%). The oil was rich in nonterpenoids (56.0%). The total flavonoid content of different extracts of the plant was found to be in the range 53.2-164.5 mg/g while the maximum amount concern to methanol extract. The antioxidant activity of the extracts was also measured by radical scavenging activity of antioxidants against free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH) method. Results showed that IC50 of the extracts of Trachyspermum ammi seed are higher than the standard synthetic antioxidants, BHT, ascorbic acid and gallic acid.

Keywords

References

  1. Omidbeigi R., 2000. Production and Processing of Medicinal Plants, Vol. I, second edition. Astan Quds Razavi Publications. [In Persian]
  2. Hopper D., 1937. Useful plants and drugs of Iran and Iraq. Field Museum of Natural Historyââ‚‌”Botany, Vol. IX, Field Museum, Chicago, IL, USA.
  3. Rechinger K.H., 1986. Sclerorhachis, in: Flora Iranica, Compositae No. 158. Edits., K.H. Rechinger and I.C. Hedge, P 47, Akademische Druck and Verlagsanstalt, Graz, Austria.
  4. Mozaffarian V., 1996. A Dictionary of Iranian plant names.FarhangMoaserPublischer, Tehran, 1st Edit., p 491.
  5. Hussein G.H., Miyashiro H., Nakamura N., Hattori M., Kakiuchi N., Shimotohno K., 2000. Inhibitory effects of Sudanese medicinal plant extracts on hepatitis C virus (HCV) protease. Phytother Res., 14, 510ââ‚‌“516.
  6. Thangam C., Dhananjayan R., 2003. Anti-inflammatory potential of the seeds of Carumcopticum Linn. Ind J Pharmacol. 34, 388ââ‚‌“391.
  7. Rasooli I., Fakoor M.H., Yadegarinia D., Gachkar L., Allameh A., Rezaei M.B., 2008. Anti Mycotoxigenic characteristics of Rosmarinus officinalis and Trachyspermum copticum L. essential oils. Int. J Food Microbiol. 122, 135ââ‚‌“139.
  8. Dashti-Rahmatabadi M.H., Hejazian S.H., Morshedi A., Rafati A., 2007. The analgesic effect of Carumcopticum extract and morphine on phasic pain in mice. J Ethnopharmacol. 109, 226ââ‚‌“228.
  9. Bera D., Lahiri D., Nag A., 2004. Novel natural antioxidant for stabilization of edible oil: the ajowan (Carum copticum) extract case. Journal of the American Oil Chemists Society JAOCS. 81, 169ââ‚‌“172.
  10. Rohman A., Riyanto S., Yuniarti N., Saputra W.R., Utami R., 2010. Antioxidant activity, total phenolic, and total flavaonoid of extracts and fractions of redfruit (PandanusconoideusLam). Int Food Res J. 17, 97-106.
  11. Chen C., Pearson M.A., Gray I.J., 1992. Effects of synthetic antioxidants (BHA, BHT and PG) on the mutagenicity of IQ-like compounds. Food Chem. 43, 177-183.
  12. Adams R.P., 2007. Identification of Essential oil Components by Gas Chromatography/Mass Spectrometry. Allured Pub. Corp., Carol Stream, IL., USA.
  13. Singleton V.L., Orthofer R., Lamuela-Raventos R.M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folinââ‚‌“Ciocalteu reagent. Meth Enzymol. 299, 152ââ‚‌“178.
  14. Mensor L.I, Menezes F.S., Leitao G.G., Reis A.S., Dos Santos T., Coube C.S., Leitao S.G., 2001. Screening of Brazilian plants extracts for antioxidants activity by use of DPPH free radical method. Phytother Res. 15, 127-130.
  15. Mohsen M.S., Ammar S.M.A., 2008. Total phenolic contents and antioxidant activity of corn tassel extracts. Food Chem. 112, 595-598.
  16. Amarowicz R., Pegg B.R., Rahimi-moghaddam P., Bar B., Weil J.A., 2003. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem. 84, 551-562.

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History

  • Receive Date: 02 December 2014
  • Revise Date: 25 October 2021
  • Accept Date: 29 October 2018

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