In this work 3, 6-bis (2-[2-sulfanyl-ethylimino)-methyl]-4-(4-nitro-phenylazo)-3-metoxy-phenol) pyridazine (2) was prepared and its antibacterial properties have studied. The structures of all the newly synthesized compounds have been comfirmed by elemental analysis, IR, 1H-NMR. Anti bacterial effects of compounds (1 and 2) have been studied. Following concentrations of materials were used: a=0.005 g/ml and b=0.01 M. Study was done on two strains of S.aureus and E.coli that experiments were done in Trypticase Soy Broth (TSB) and Nutrien Agar (TSB; Difco Laboratory). It is likely that these synthetic materials exert higher antibacterial effect on gram positive bacteria in comparison with gram negative.