Study of Antioxidant and Free Radical Scavenging Activities of Cotoneaster medicus and Glycyrrhiza glabra Plants


1 Department of Chemistry , Mashhad Branch , Islamic Azad university, Mashhad, Iran

2 Young Researchers Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran


Extracts of Cotoneaster medicus, Glycyrrhiza glabra, as endemic plants of Iran, along with mixture of them were investigated for their antioxidant activities using 2, 2- diphenyl-1-picrylhidrazyl (DPPH) reagent. UV-Vis spectrophotometry method was used to evaluate the ability of Cotoneaster and Glycyrrhiza glabra antioxidant to scavenge DPPH radical. The kinetic parameters such as rate constant and activation energy in experimental conditions were calculated. The rate constants of the H atom abstraction by DPPH (k1), in the presence of C. medicus and G. glabra antioxidant were obtained under pseudo-first-order conditions at different temperatures. The order in DPPH radical-scavenging was: mixture of C. medicus and G. glabra > C. medicus > G.  glabra plants. The numerical values of activation energy were found to be 45.84 kJ.mol-1for G. glabra and 62.02kJ.mol-1 for C. medicus.


Al-Dabbas M., Suganuma T., Kitahara K.,
X.Hou D., 2006. antioxidant and antibacterial
activities of Varthemia iphionoides Boiss.
Extracts. J Ethnopharmacol.108: 287-293.
Tsa R., Deng Z., 2004. Separation procedures
for naturally occurring antioxidant
phytochemicals. J. Chromatogr. B 812: 85-99.
Hayet E., Maha M., Samia A., Mata M., Gros
P., Raida H., Ali M. M., Mohamed A. S., Gutmann
L., Mighri Z., Mahjoub A., 2008. Antimicrobial,
antioxidant, and antiviral activities of Retama
raetam (Forssk.) Webb flowers growing in
Tunisia.World J. Microbiol. Biotechnol. 24: 2933-
Burns J., Gardner P.T., Matthews D., Duthie
G.G., Lean M.E., Crozier A., 2001. Extraction of
phenolics and changes in antioxidant activity of
red wines during vinification. J.
Agric. Food Chem. 49: 5797-5808.
Smith M. A., Perry G., Richey P.L., Sayre
L.M., Anderson V.E., Beal M.F., 1996. Oxidative
damage in Alzheimerââ‚‌™s. Nature 382: 120-121.
Diaz M., Frei B., Vita J.A., Keaney J.F., 1997.
Antioxidants and atherosclerotic heart disease. N.
Engl. J. Med. 337: 408-416.
Aruoma O. I., 1998. Free radicals, oxidative
stress, and antioxidants in human health and
disease. J. Am. Oil Chem.Soc. 75: 199-212.
Lim Y. Y., Murtijaya J., 2007. Antioxidant
properties of Phyllanthus amarus extracts as
affected by different drying methods. LWT-Food
Sci. Technol. 40:1664-1669.
Scalbert A., Manach C., Morand C., Remesy C.,
Dietary Polyphenols and the Prevention of
Diseases. Crit. Rev. Food Sci. Nutr. 45: 287-306.
Wang K., Pan Y., Wang H., Zhang Y., Lei Q.,
Zhu Z., 2010. Med. Chem. Res.
: 166-176.
Gulcin I., Elias R., Gepdiremen A., Taoubi K.,
Koksal E., 2009. Antioxidant secoiridoids from
fringe tree (Chionanthus virginicus L.).Wood Sci.
Technol. 43: 195-212.
Dickinson T. A., Arsenault M. P., 2007.
Phylogeny of subtribe Pyrinae (formerly the
Maloideae, Rosaceae): limited resolution of a
complex evolutionary history. -Pl. Syst. Evol. 266:
ââ‚‌“ 145.
Mabberley D. J., 1997. The Plant Book, second
edition. -Cambridge University Press.
Tamir S., Eizenberg M., Somjen D., 2001.
Estrogenlike activity of glabrene and other
constituents isolated from licorice root. J Steroid
Biochem Mol Bio .78:291-298.
Yang Me D., Wang Me Q., Ke Be L., Jiang Be
J., Ying T., 2007. Antioxidant activities of various
extracts of lotus (Nelumbo nuficera Gaertn)
rhizome. Asia Pacific Journal of Clinical Nutrition,
: 158-163.
Ilhami G., Ercan B., Hilal S., Ehitog Lu.,
Mine B., Ahmet C. G., 2010. Polyphenol contents
and antioxidant activity of lyophilized aqueous
extract of propolis from Erzurum, Turkey. Food
and Chemical Toxicology, 48; 2227ââ‚‌“2238.
Jing D., Wangyuan C., Guangzhong Y., 2011.
A novel antioxidant activity index (AAU) for
natural products using the DPPH assay. Food
Chemistry. 125 ; 1430-1435
Bolvar A., Evalos C. C., Luiscisneros Z.,
Stoichiometric and Kinetic Studies of
Phenolic Antioxidants from Andean Purple Corn
and Red-Fleshed Sweetpotato. journal of
Agriculcural and food chemistry,51;3313−3319.
Hristea E., Caproiu M., Pencu G., Hillebrand
M., Constantinescu T., Alexandru T., 2006.
Reaction of 2,2-Diphenyl-1-picrylhydrazyl with
HO·, O2·ââ‚‌“, HOââ‚‌“ ,and HOOââ‚‌“ Radicals and Anions.
Int. J. Mol. Sci. 7: 130-143.