Kinetic Study of Free Radicals Scavenging by Saffron Petal Extracts



Saffron petal is the main by-product of saffron processing which is produced in large amounts, annually. The objectives of this study were to study the antioxidant activity and free radical-scavenging effects of saffron petal extracts. The ability of saffron petal to act as an antioxidant using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free-radical method was investigated by applying the Uv–Vis spectrometry. The Uv–Vis spectra of reaction mixtures in acetonitrile revealed that saffron petal has a considerable effect on scavenging free radical. Kinetic studies were conducted by measuring the disappearance of DPPH in acetonitrile over the wavelength range of 515-522 nm under pseudo-first-order conditions at 37oC. Furthermore, the pseudo first order rate constants were determined


  1. Scott, G; 1993, Atmospheric oxidation and antioxidants, Elsevier Amsterdam., 1, 38.
  2. Halliwell, B; 1999, Establishing the Significance and Optimal Intake of Dietary Antioxidants, Nutrition Review, 57, 104.
  3. Ames, B., Shigenaga, M and Hagen T; 1999, Oxidants, antioxidants, and the degenerative diseases of aging, Proceedings of the National Academy of Sciences, 90, 7915.
  4. Halliwell, B; 1994, Formation of hydroxyl radicals in biological systems, Nutrition Reviews, 52, 253.
  5. Rice-Evans, C., Miller. N and Paganga. G; 1997, Antioxidant properties of phenolic compounds, Trends in Plant Science, 4, 304.
  6. Vendemiale. G., Grattagliano. I and Altomare. E; 1999, An update on the role of free radicals and antioxidant defense in human disease, International Journal of Clinical and Laboratory Research, 29, 49.
  7. Abou-Seif. M and Youssef A; 2004, Evaluation of some biochemical changes in diabetic patients, Clinical Chemical Acta, 346, 161.
  8. Freitas. P., Filipe. P and A. Rodrigo; 1997, inhibition on glutathione redox status in erythrocytes Diabetes Research and Clinical Practice, 36, 71.
  9. Momen Herav. M., Haghi B., Morsali. A., Ardalan. P., Ardalan. T; 2012, Kinetic study of DPPH scavenging in the presence of mixture of Zinc and Vitamin C as an antioxidant, Journal of Chemical Health Risks, 2,43-50.
  10. Emadi. B., Saiedirad. M and Mahmoodi. A; 2008, In Proceedings of 4th International Conference on Innovations in Food Processing Technology and Engineering, Bangkok,Thailand. Paper .
  11. Gresta. F., Avola. G., Lombardo. G., Siracusa. L and Ruberto ; 2009, 5 Analysis of flowering, stigmas yield and qualitative traits of saffron (Crocus sativus L.) as affected by environmental conditions, Scientia Horticulture,119, 320.
  12. Fatehi. M., Rashidabady. T and Fatehi-Hassanabad. Z; 2003, Effects of Crocus sativus petals' extract on rat blood pressure and on responses induced by electrical field stimulation in the rat isolated vas deferens and guinea-pig ileum ,Journal of Ethnopharmacology, 84, 199.
  13. Hosseinzadeh. H and Khosrava V; 2002, Anticonvulsant effects aqueous and ethanolic extracts of Crocus sativus stigmas in mice, Archives of Iranian Medicine, 5, 44.
  14. Akhondzadeh. S; 2004, Comparison of Crocus sativus L. and imipramine in the treatment of mild to moderate depression: A pilot double-blind randomized trial [ISRCTN45683816], Alternative Medicine, 4,12.
  15. Martin. G; 2002, foodchemtox Methods of in vitro toxicology, Food and Chemical Toxicology, 40,959.
  16. Hosseinzadeh. H and Younesi. H; 2002, Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice, BMC Pharmacology, 2, 7.
  17. A. Ahmad; 2005, Neuroprotection by crocetin in a hemi-parkinsonian rat model., Biochemistry and Behavior, 81,805.
  18. Salomi. M; 1991, (Crocus sativus) on chemical carcinogenesis in mice, mice Nutrition and Cancer ,16 ,67.
  19. Abdullaev. F; 2002, Cancer Chemopreventive and Tumoricidal Properties of Saffron (Crocus sativus L.),Experimental Biology and Medicine, 227, 20.
  20. Abdullaev. F L. Riverón-Negrete, H. Caballero-Ortega, J. Manuel Hernández, I. Pérez-López, R. Pereda-Miranda, J. J. Espinosa-Aguirre; 2003, Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron ( Crocus sativus L), Toxicology in vitro, 17, 731.
  21. Nair. S., Pannikar, B.,Panikkar, K. R; 1991, Antitumour activity of saffron (Crocus sativus) Cancer Letter, 57,109.
  22. Nair. S., Panikkar. K and Parathod. R; 1993, Cancer Biotherapy, 8,339.
  23. Nair. S; (1994) International Journal of Pharmacology, 32,105.
  24. Premkumar. K; 2003, Protective effects of saffron (Crocus sativus Linn.) on genotoxins-induced oxidative stress in Swiss albino mice, Phytother Research, 17, 614.
  25. Ahmad Wani. B , Khan Rouf Hamza. A and Mohiddin. F; 2011, Saffron: A repository of medicinal properties, Journal of Medicinal Plants Research, 5, 2131.
  26. Yang Me. D., Wang Me. Q., Ke Be. L., Jiang Be. J and Ying. T; 2007, Antioxidant activities of various extracts of lotus (Nelumbo nuficera Gaertn) rhizome, Asia Pacific Journal of Clinical
  27. Nutrition, 16, 158.
  28. Hristea. E., Caproiu. M., Pencu. G., Hillebrand. M, Constantinescu. T and T. Alexandru; 2006, Reaction of 2,2-Diphenyl-1-picrylhydrazyl with HO·, O2·ââ‚‌“, HOââ‚‌“ ,and HOOââ‚‌“ Radicals and Anions, Int. J. Mol. Sci, 7, 130.
  29. Lu-Ming. L., Wei-Shuen. L .,Wei-Lin. C and Andrew. Y; 2010, Oxidation of Catechols and Flavonoids by DPPH, Journal of the Chinese Chemical Society, 57, 883.
  30. Kutlu. T., Durmaz. J., Ates. B., Yilmaz. I and Cetin. S; 2011, Turkish Journal of Biology, 35, 103.
  31. Wojdyło. A, Oszmian´ski. J and Czemerys. R; 2007, Antioxidant activity and phenolic compounds in 32 selected herbs, Food Chemistry, 105 , 940.
  32. Kumar. S., Priyadarsini. K and Sainis. K; 2002, Free radical scavenging activity of vanillin and o-vanillin using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical Redox Report, 7, 35.