The Effects of Environmental Pollution with Heavy Metals in Frequency of Micronuclei in Epithelial Buccal Cells of Human Population in Mitrovica



The purpose of this study was evaluation of genotoxic effects of environmental pollution with heavy metals in inhabitants of Mitrovica town (which is located close to smelter “Treça” down closed). In this study, 55 inhabitants (males) from Mitrovica town and 20 (males) control subjects with similar mean ages and smoking prevalence were enrolled for analysis of micronuclei frequency in epithelial buccal cells. The subjects of Mitrovica town showed significant increase micronuclei frequency (p< 0.001) in epithelial buccal cells compared to controls and with respect to their smoking habits. The current study suggests that chronic exposure to pollution with heavy metals could lead to increase of DNA damage.


  1. Silbergeld E.K. 2008. Gene: Environmental interactions in metal toxicity. Proceedings of 14th International Conference on Heavy Metals in the Environment. 273-275.
  2. Nersesyan A.K., Vardazaryan N.S., Gevorgyan A.L. Arutyunyan R.M. 2002. Micronucleus level in exfoliated buccal murosa cells of kancer patients. Archive of oncology. 10: 35-36.
  3. Fenech M., Holland N., Chang W.P., Zeiger E., Bonassi S. 1999. The Human MicroNucleus Project- an internacional collaborative study on the use of the micronucleus technique for measuring DNA damave in humans. Mutation Research.. 428:271 283.
  4. Tolbert,P.E., Shy,C.M. and Allen,J.W. 1992. Micronuclei and other nuclear bnormalities in buccal smears: methods and development. Mutat. Res.,271, 69-77.
  5. Grandjean P., Wulf H., Nebuhr. 1983. Sister chromatide Exchange in lead-exposed worcers,Environ Res. 199-204.
  6. Popovac D, Graziano J, Seaman C, Kaul B, Colakovic B, Popovac T., Osmani, I., Haxhiu, M., Begraca, M, Bozovic Z, Mikic M. 1982. Evaluated blood lead in a population near a lead smelter in Kosovo, Yugoslavia. Arch. Environ. Health, 37, 19-23.
  7. Devi K.R., Madhavi D., Kumari J.K. 2011. Nuclear anomalies in exfoliated buccal cells od occupationaly lead exposed population. International Journal of Pharma and Bio Sciences. 2. 4: B 710-B712.
  8. Alexander A.C., Sonia S.P. Rchardo M. 2001. Occupational exposure to lead and induction of geentic damave. Environ Health Per. 2001; 109 (3): 295-298.
  9. Hamurcu Z., Donmez H., Saraymen R. and Demirtas H. 2001. Micronucleus Frequencies in workers Exposed to Lead, Zinc and Cadmium. Biological Trace Element Reserch.; 83. 97-102
  10. Martino-Roth M.G., Viégas J., Amaral M., Oliveira L., Ferreira F. L. S and Erdtmann B. 2002. Evaluation of genotoxicity through micronuclei test in workers of car and battery repair garages. Genetics and Molecular Biology, (25); 495-500.
  11. Letaj K.Rr., Elezaj I.R., Selimi Q.I. , Kurteshi K.H. 2011. The Genotoxic Effects of Environmental Pollution with Heavy Metals in cat (Fesils domesticus). Proceedings of 15th International Conference on Heavy Metals in the Environment. 661.
  12. Çelik, A., ÇavaÅŸ, T. and Ergene-Gözükara, S. 2003. Cytogenetic Biomonitoring in petrol stacion attention Micronucleus test in exfoliated buccal cells. Mutagenesis. 2003; 18: 41ââ‚‌”421.
  13. International Agency For Reserch on Cancer (IARC) 1980. Monographs on the Evaluation of the Carcinogenic Risc to Humans-Some Metals and metallic Compounds, WHO, Lyon, 23,; 1-210,325-415.