Kinetic study of DPPH scavenging in the presence of mixture of Zinc and Vitamin C as an antioxidant

Authors

1 Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran

2 Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran, young researcher club

Abstract

Reactions of free radicals and reactive oxygen species (ROS) with biological molecules in vivo play an important physiological role in many diseases. 2,2-diphenyl-1-picrylhydrazyl (DPPH) is a stable free radical and often used as a routine reagent to evaluate the antioxidant capacity of an antioxidant. This study was undertaken to investigate the free radical-scavenging and antioxidant activities of Zinc, Vitamin C and mixture of them. UV-Vis spectrophotometry method was used to evaluate the ability of Zinc, Vitamin C and mixture of them to scavenge DPPH radical. The kinetic parameters such as rate constant and activation energy in experimental conditions were calculated. The rate constants of the H atom abstraction by DPPH (), in the presence of Zinc, Vitamin C and mixture of them were obtained (0.4209, 2.092 and 1.82 min-1) respectively, under pseudo-first-order conditions at 25 oC.

Keywords

References

  1. Aruoma, O. I. 2003. Methodological
  2. considerations for characterizing potential
  3. antioxidant actions of bioactive components in
  4. plant foods. Mutation. Research. 9: 523-524.
  5. He, Y. Y and D. P. Häder. 2002. Reactive oxygen
  6. species and UV-B: effect on Cyanobacteria.
  7. Photochem. Photobiol. Sci. 1: 729-736.
  8. Jacobi, R.A., B. J Burri, 1996. Oxidative damage
  9. and defense. Am. J. Clin. Nutr., 63, 985-990.
  10. Apel, K and H. Hirt. 2004. Reactive oxygen
  11. species: metabolism, oxidative stress and signal
  12. transduction. Annu. Rev. Plant. Biol. 55: 373-399.
  13. Aruoma, O.I. 1998. Free radicals, oxidative stress,
  14. and antioxidants in human health and disease. J.
  15. Am. Oil Chem. Soc. 75: 199-212.
  16. Prior, R. L and G. Cao. 2000. Antioxidant
  17. phytochemicals in fruits and vegetables. Diet and
  18. health implications. Hort. Sci. 35: 588-592.
  19. Pryor, W.A. 1991. The antioxidant nutrient and
  20. disease prevention-What do we know and what
  21. we need to find out. Am. J. Clin. Nutr, 53: 391-
  23. Cook, N. C and S. Samman. 1996. Flavonoids:
  24. Chemistry, metabolism, cardioprotective effects,
  25. and dietary sources. J.Nutr. Biochem. 7: 66-76.
  26. Lien, E. J; S. Ren; S. H. H. Bui and R.Wang.
  27. Quantitative structure activity analysis of
  28. phenolic antioxidants. Free. Rad. Bio. Med. 26:
  29. -294.
  30. Rice-Evans, C.A; N.J. Miller and G. Paganga.
  31. Antioxidant properties of phenolic
  32. compounds. Trends. Plant. Sci. 2: 152-159.
  33. Mashimbye, M. J; F. Mudau; P. Soundy and T.
  34. Van Ree. 2006. A new flavonol fromAthrixia
  35. phylicoides (Bush tea). S. Afri. J. Chem. 59: 1-2.
  36. Mcgaw, L. J; V. Steenkamp and J. N. Eloff, 2007.
  37. Evaluation of Athrixia bush tea for cytotoxicity,
  38. antioxidant activity, caffeine content and presence
  39. of pyrrolizidine alkaloids. J. Ethnopharmacol.
  40. : 16-22.
  41. Scalbert, A and G. Williamson. 2000. Dietary
  42. intake and bioavailability of polyphenols. J. Nutr.
  43. : 2073.
  44. Rice-Evans, C.A; N.J. Miller and G. Paganga.
  45. Structure-antioxidant activity relationships
  46. of flavonoids and phenolic acids. Free. Radical.
  47. Biol. Med. 20: 933ââ‚‌“956.
  48. Phippen, W.B and J. E. Simon. 2000.
  49. Anthocyanin inheritance and instability in purple
  50. basil (Ocimum basilicum L.). J. Hered. 91: 289ââ‚‌“
  52. Phippen, W.B and J. E. Simon. 1998.
  53. Anthocyanins in basil (Ocimum basilicum L.). J.
  54. Agr. Food Chem. 46: 1734-1738.
  55. Blois, M. S. 1958. Antioxidant determinations by
  56. the use of a stable free radical. Nature. 181: 1199-
  58. Anderson, D; B. J. Phillips; T. Yu; A. J. Eduards;
  59. R. Ayesh and K. R. Butterworth. 1997. The effect
  60. of DPPH radical. Biochem. Pharmacol. 56: 213-
  62. Johnson, C; S. Kaviarasan; H. Naik; R.
  63. Gangabhagirathi; C. V. Anuradha and K. I.
  64. Priyadarsini. 2007. In vitro studies on antiradical
  65. and antioxidant activities of fenugreek (Trigonella
  66. foenum graecum) seeds. Food. Chem. 103: 31-37.
  67. Kumar, S. S; K. I. Priyadarsini and K. B. Sainis.
  68. Free radical scavenging activity of vanillin
  69. and O-vanillin using 1,1-diphenyl-2-
  70. picrylhydrazyl (DPPH) radical. Redox. Rep. 7:
  71. -40.
  72. Wang, M; Y. Jin and C. T. Ho. 1999. Evaluation
  73. of resveratrol derivatives as potential antioxidants
  74. and identification of a reaction product of
  75. resveratrol and 2, 2- diphenyl-1-picrylhydrazyl
  76. radical. J. Agr. Food. Chem. 47: 3974-3977.
  77. Yokozawa, T; C. P. Chen; E. Dong; T. Tanaka; G.
  78. I. Nonaka and I. Nishioka. 1998. Study on the
  79. inhibitory effect of tannins and flavonoids against
  80. the 1, 1-diphenyl-2-picrylhydrazyl radical.
  81. Biochem. Pharmacol. 56: 213-222.
  82. Gazi, M. R; K. Kanda; M. Yasuda and F. Kato.
  83. Optimisation of cultural conditions and
  84. some properties of radical scavenging substances
  85. from sporobolomyces salmonicolor Pak. J. Biol.
  86. Sci. 7: 1365-1370.
  87. Molyneux. P. 2004. The use of the stable free
  88. radical diphenylpicryl-hydrazyl (DPPH) for
  89. estimating antioxidant activity. Songklanakarin. J.
  90. Sci. Technol. 26: 211-219.
  91. Pavlov, A; P. Kovatcheva; V. Georgiev; I. Koleva
  92. and M Ilieva. 2002. Biosynthesis and radical
  93. scavenging activity of betalains during the
  94. cultivation of red beet (Beta vulgaris) hairy root
  95. cultures. Z. Naturforsch. 57c: 640-644.
  96. Bray, T. M and W. J. Bettger. 1990. The
  97. physiologic role of zinc as an antioxidant. Free.
  98. Radic. Biol. Med. 8: 281-291
Journal of Chemical Health Risks

Volume 2, Issue 2
Spring 2012

Files

History

  • Receive Date: 06 August 2012
  • Revise Date: 18 February 2020
  • Accept Date: 29 October 2018

Share

How to cite

Statistics