Kinetic study of DPPH scavenging in the presence of mixture of Zinc and Vitamin C as an antioxidant

Authors

1 Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran

2 Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran, young researcher club

Abstract

Reactions of free radicals and reactive oxygen species (ROS) with biological molecules in vivo play an important physiological role in many diseases. 2,2-diphenyl-1-picrylhydrazyl (DPPH) is a stable free radical and often used as a routine reagent to evaluate the antioxidant capacity of an antioxidant. This study was undertaken to investigate the free radical-scavenging and antioxidant activities of Zinc, Vitamin C and mixture of them. UV-Vis spectrophotometry method was used to evaluate the ability of Zinc, Vitamin C and mixture of them to scavenge DPPH radical. The kinetic parameters such as rate constant and activation energy in experimental conditions were calculated. The rate constants of the H atom abstraction by DPPH (), in the presence of Zinc, Vitamin C and mixture of them were obtained (0.4209, 2.092 and 1.82 min-1) respectively, under pseudo-first-order conditions at 25 oC.

Keywords

References

  1. Aruoma, O. I. 2003. Methodological
  2. considerations for characterizing potential
  3. antioxidant actions of bioactive components in
  4. plant foods. Mutation. Research. 9: 523-524.
  5. He, Y. Y and D. P. Häder. 2002. Reactive oxygen
  6. species and UV-B: effect on Cyanobacteria.
  7. Photochem. Photobiol. Sci. 1: 729-736.
  8. Jacobi, R.A., B. J Burri, 1996. Oxidative damage
  9. and defense. Am. J. Clin. Nutr., 63, 985-990.
  10. Apel, K and H. Hirt. 2004. Reactive oxygen
  11. species: metabolism, oxidative stress and signal
  12. transduction. Annu. Rev. Plant. Biol. 55: 373-399.
  13. Aruoma, O.I. 1998. Free radicals, oxidative stress,
  14. and antioxidants in human health and disease. J.
  15. Am. Oil Chem. Soc. 75: 199-212.
  16. Prior, R. L and G. Cao. 2000. Antioxidant
  17. phytochemicals in fruits and vegetables. Diet and
  18. health implications. Hort. Sci. 35: 588-592.
  19. Pryor, W.A. 1991. The antioxidant nutrient and
  20. disease prevention-What do we know and what
  21. we need to find out. Am. J. Clin. Nutr, 53: 391-
  22. Cook, N. C and S. Samman. 1996. Flavonoids:
  23. Chemistry, metabolism, cardioprotective effects,
  24. and dietary sources. J.Nutr. Biochem. 7: 66-76.
  25. Lien, E. J; S. Ren; S. H. H. Bui and R.Wang.
  26. Quantitative structure activity analysis of
  27. phenolic antioxidants. Free. Rad. Bio. Med. 26:
  28. -294.
  29. Rice-Evans, C.A; N.J. Miller and G. Paganga.
  30. Antioxidant properties of phenolic
  31. compounds. Trends. Plant. Sci. 2: 152-159.
  32. Mashimbye, M. J; F. Mudau; P. Soundy and T.
  33. Van Ree. 2006. A new flavonol fromAthrixia
  34. phylicoides (Bush tea). S. Afri. J. Chem. 59: 1-2.
  35. Mcgaw, L. J; V. Steenkamp and J. N. Eloff, 2007.
  36. Evaluation of Athrixia bush tea for cytotoxicity,
  37. antioxidant activity, caffeine content and presence
  38. of pyrrolizidine alkaloids. J. Ethnopharmacol.
  39. : 16-22.
  40. Scalbert, A and G. Williamson. 2000. Dietary
  41. intake and bioavailability of polyphenols. J. Nutr.
  42. : 2073.
  43. Rice-Evans, C.A; N.J. Miller and G. Paganga.
  44. Structure-antioxidant activity relationships
  45. of flavonoids and phenolic acids. Free. Radical.
  46. Biol. Med. 20: 933ââ‚‌“956.
  47. Phippen, W.B and J. E. Simon. 2000.
  48. Anthocyanin inheritance and instability in purple
  49. basil (Ocimum basilicum L.). J. Hered. 91: 289ââ‚‌“
  50. Phippen, W.B and J. E. Simon. 1998.
  51. Anthocyanins in basil (Ocimum basilicum L.). J.
  52. Agr. Food Chem. 46: 1734-1738.
  53. Blois, M. S. 1958. Antioxidant determinations by
  54. the use of a stable free radical. Nature. 181: 1199-
  55. Anderson, D; B. J. Phillips; T. Yu; A. J. Eduards;
  56. R. Ayesh and K. R. Butterworth. 1997. The effect
  57. of DPPH radical. Biochem. Pharmacol. 56: 213-
  58. Johnson, C; S. Kaviarasan; H. Naik; R.
  59. Gangabhagirathi; C. V. Anuradha and K. I.
  60. Priyadarsini. 2007. In vitro studies on antiradical
  61. and antioxidant activities of fenugreek (Trigonella
  62. foenum graecum) seeds. Food. Chem. 103: 31-37.
  63. Kumar, S. S; K. I. Priyadarsini and K. B. Sainis.
  64. Free radical scavenging activity of vanillin
  65. and O-vanillin using 1,1-diphenyl-2-
  66. picrylhydrazyl (DPPH) radical. Redox. Rep. 7:
  67. -40.
  68. Wang, M; Y. Jin and C. T. Ho. 1999. Evaluation
  69. of resveratrol derivatives as potential antioxidants
  70. and identification of a reaction product of
  71. resveratrol and 2, 2- diphenyl-1-picrylhydrazyl
  72. radical. J. Agr. Food. Chem. 47: 3974-3977.
  73. Yokozawa, T; C. P. Chen; E. Dong; T. Tanaka; G.
  74. I. Nonaka and I. Nishioka. 1998. Study on the
  75. inhibitory effect of tannins and flavonoids against
  76. the 1, 1-diphenyl-2-picrylhydrazyl radical.
  77. Biochem. Pharmacol. 56: 213-222.
  78. Gazi, M. R; K. Kanda; M. Yasuda and F. Kato.
  79. Optimisation of cultural conditions and
  80. some properties of radical scavenging substances
  81. from sporobolomyces salmonicolor Pak. J. Biol.
  82. Sci. 7: 1365-1370.
  83. Molyneux. P. 2004. The use of the stable free
  84. radical diphenylpicryl-hydrazyl (DPPH) for
  85. estimating antioxidant activity. Songklanakarin. J.
  86. Sci. Technol. 26: 211-219.
  87. Pavlov, A; P. Kovatcheva; V. Georgiev; I. Koleva
  88. and M Ilieva. 2002. Biosynthesis and radical
  89. scavenging activity of betalains during the
  90. cultivation of red beet (Beta vulgaris) hairy root
  91. cultures. Z. Naturforsch. 57c: 640-644.
  92. Bray, T. M and W. J. Bettger. 1990. The
  93. physiologic role of zinc as an antioxidant. Free.
  94. Radic. Biol. Med. 8: 281-291

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History

  • Receive Date: 06 August 2012
  • Revise Date: 19 May 2021
  • Accept Date: 29 October 2018

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