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Momen Heravi, M., Haghi, B., Morsali, A., Ardalan, P., Ardalan, T. (2012). Kinetic study of DPPH scavenging in the presence of mixture of Zinc and Vitamin C as an antioxidant. Journal of Chemical Health Risks, 2(2), -. doi: 10.22034/jchr.2012.543994
M. Momen Heravi; B. Haghi; A. Morsali; P. Ardalan; T. Ardalan. "Kinetic study of DPPH scavenging in the presence of mixture of Zinc and Vitamin C as an antioxidant". Journal of Chemical Health Risks, 2, 2, 2012, -. doi: 10.22034/jchr.2012.543994
Momen Heravi, M., Haghi, B., Morsali, A., Ardalan, P., Ardalan, T. (2012). 'Kinetic study of DPPH scavenging in the presence of mixture of Zinc and Vitamin C as an antioxidant', Journal of Chemical Health Risks, 2(2), pp. -. doi: 10.22034/jchr.2012.543994
Momen Heravi, M., Haghi, B., Morsali, A., Ardalan, P., Ardalan, T. Kinetic study of DPPH scavenging in the presence of mixture of Zinc and Vitamin C as an antioxidant. Journal of Chemical Health Risks, 2012; 2(2): -. doi: 10.22034/jchr.2012.543994

Kinetic study of DPPH scavenging in the presence of mixture of Zinc and Vitamin C as an antioxidant

Article 6, Volume 2, Issue 2, Summer 2012  XML PDF (390.28 K)
DOI: 10.22034/jchr.2012.543994
Authors
M. Momen Heravi 1; B. Haghi1; A. Morsali1; P. Ardalan2; T. Ardalan2
1Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran
2Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran, young researcher club
Receive Date: 06 August 2012,  Revise Date: 18 February 2019,  Accept Date: 29 October 2018 
Abstract
Reactions of free radicals and reactive oxygen species (ROS) with biological molecules in vivo play an important physiological role in many diseases. 2,2-diphenyl-1-picrylhydrazyl (DPPH) is a stable free radical and often used as a routine reagent to evaluate the antioxidant capacity of an antioxidant. This study was undertaken to investigate the free radical-scavenging and antioxidant activities of Zinc, Vitamin C and mixture of them. UV-Vis spectrophotometry method was used to evaluate the ability of Zinc, Vitamin C and mixture of them to scavenge DPPH radical. The kinetic parameters such as rate constant and activation energy in experimental conditions were calculated. The rate constants of the H atom abstraction by DPPH (), in the presence of Zinc, Vitamin C and mixture of them were obtained (0.4209, 2.092 and 1.82 min-1) respectively, under pseudo-first-order conditions at 25 oC.
Keywords
Antioxidant; DPPH; Kinetic; Free radical; UV-Vis spectrometry
References
  1. Aruoma, O. I. 2003. Methodological
  2. considerations for characterizing potential
  3. antioxidant actions of bioactive components in
  4. plant foods. Mutation. Research. 9: 523-524.
  5. He, Y. Y and D. P. Häder. 2002. Reactive oxygen
  6. species and UV-B: effect on Cyanobacteria.
  7. Photochem. Photobiol. Sci. 1: 729-736.
  8. Jacobi, R.A., B. J Burri, 1996. Oxidative damage
  9. and defense. Am. J. Clin. Nutr., 63, 985-990.
  10. Apel, K and H. Hirt. 2004. Reactive oxygen
  11. species: metabolism, oxidative stress and signal
  12. transduction. Annu. Rev. Plant. Biol. 55: 373-399.
  13. Aruoma, O.I. 1998. Free radicals, oxidative stress,
  14. and antioxidants in human health and disease. J.
  15. Am. Oil Chem. Soc. 75: 199-212.
  16. Prior, R. L and G. Cao. 2000. Antioxidant
  17. phytochemicals in fruits and vegetables. Diet and
  18. health implications. Hort. Sci. 35: 588-592.
  19. Pryor, W.A. 1991. The antioxidant nutrient and
  20. disease prevention-What do we know and what
  21. we need to find out. Am. J. Clin. Nutr, 53: 391-
  23. Cook, N. C and S. Samman. 1996. Flavonoids:
  24. Chemistry, metabolism, cardioprotective effects,
  25. and dietary sources. J.Nutr. Biochem. 7: 66-76.
  26. Lien, E. J; S. Ren; S. H. H. Bui and R.Wang.
  27. Quantitative structure activity analysis of
  28. phenolic antioxidants. Free. Rad. Bio. Med. 26:
  29. -294.
  30. Rice-Evans, C.A; N.J. Miller and G. Paganga.
  31. Antioxidant properties of phenolic
  32. compounds. Trends. Plant. Sci. 2: 152-159.
  33. Mashimbye, M. J; F. Mudau; P. Soundy and T.
  34. Van Ree. 2006. A new flavonol fromAthrixia
  35. phylicoides (Bush tea). S. Afri. J. Chem. 59: 1-2.
  36. Mcgaw, L. J; V. Steenkamp and J. N. Eloff, 2007.
  37. Evaluation of Athrixia bush tea for cytotoxicity,
  38. antioxidant activity, caffeine content and presence
  39. of pyrrolizidine alkaloids. J. Ethnopharmacol.
  40. : 16-22.
  41. Scalbert, A and G. Williamson. 2000. Dietary
  42. intake and bioavailability of polyphenols. J. Nutr.
  43. : 2073.
  44. Rice-Evans, C.A; N.J. Miller and G. Paganga.
  45. Structure-antioxidant activity relationships
  46. of flavonoids and phenolic acids. Free. Radical.
  47. Biol. Med. 20: 933ââ‚‌“956.
  48. Phippen, W.B and J. E. Simon. 2000.
  49. Anthocyanin inheritance and instability in purple
  50. basil (Ocimum basilicum L.). J. Hered. 91: 289ââ‚‌“
  52. Phippen, W.B and J. E. Simon. 1998.
  53. Anthocyanins in basil (Ocimum basilicum L.). J.
  54. Agr. Food Chem. 46: 1734-1738.
  55. Blois, M. S. 1958. Antioxidant determinations by
  56. the use of a stable free radical. Nature. 181: 1199-
  58. Anderson, D; B. J. Phillips; T. Yu; A. J. Eduards;
  59. R. Ayesh and K. R. Butterworth. 1997. The effect
  60. of DPPH radical. Biochem. Pharmacol. 56: 213-
  62. Johnson, C; S. Kaviarasan; H. Naik; R.
  63. Gangabhagirathi; C. V. Anuradha and K. I.
  64. Priyadarsini. 2007. In vitro studies on antiradical
  65. and antioxidant activities of fenugreek (Trigonella
  66. foenum graecum) seeds. Food. Chem. 103: 31-37.
  67. Kumar, S. S; K. I. Priyadarsini and K. B. Sainis.
  68. Free radical scavenging activity of vanillin
  69. and O-vanillin using 1,1-diphenyl-2-
  70. picrylhydrazyl (DPPH) radical. Redox. Rep. 7:
  71. -40.
  72. Wang, M; Y. Jin and C. T. Ho. 1999. Evaluation
  73. of resveratrol derivatives as potential antioxidants
  74. and identification of a reaction product of
  75. resveratrol and 2, 2- diphenyl-1-picrylhydrazyl
  76. radical. J. Agr. Food. Chem. 47: 3974-3977.
  77. Yokozawa, T; C. P. Chen; E. Dong; T. Tanaka; G.
  78. I. Nonaka and I. Nishioka. 1998. Study on the
  79. inhibitory effect of tannins and flavonoids against
  80. the 1, 1-diphenyl-2-picrylhydrazyl radical.
  81. Biochem. Pharmacol. 56: 213-222.
  82. Gazi, M. R; K. Kanda; M. Yasuda and F. Kato.
  83. Optimisation of cultural conditions and
  84. some properties of radical scavenging substances
  85. from sporobolomyces salmonicolor Pak. J. Biol.
  86. Sci. 7: 1365-1370.
  87. Molyneux. P. 2004. The use of the stable free
  88. radical diphenylpicryl-hydrazyl (DPPH) for
  89. estimating antioxidant activity. Songklanakarin. J.
  90. Sci. Technol. 26: 211-219.
  91. Pavlov, A; P. Kovatcheva; V. Georgiev; I. Koleva
  92. and M Ilieva. 2002. Biosynthesis and radical
  93. scavenging activity of betalains during the
  94. cultivation of red beet (Beta vulgaris) hairy root
  95. cultures. Z. Naturforsch. 57c: 640-644.
  96. Bray, T. M and W. J. Bettger. 1990. The
  97. physiologic role of zinc as an antioxidant. Free.
  98. Radic. Biol. Med. 8: 281-291
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